The yeast nucleoporin rip1p contributes to multiple export pathways with no essential role for its FG-repeat region.
The yeast nucleoporin rip1p contributes to multiple export pathways with no essential role for its FG-repeat region.
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酵母核孔蛋白 rip1p 有助于多种输出途径，但其 FG 重复区域没有重要作用。
DOI：
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发表时间：
1997
影响因子：
10.5
通讯作者：
Michael Rosbash
中科院分区：
文献类型：
--
作者：
Françoise Stutz;Julia Kantor;Dong Zhang;Terri McCarthy;Megan Neville;Michael Rosbash
在酵母双杂交试验中，酵母Rip 1蛋白（Rip 1 p）的FG重复结构域最初被鉴定为HIV-1 Rev蛋白核输出信号（内斯）的可能靶点。Rip 1 p是不必要的，与核孔复合物，并在结构上相关的FG-核孔蛋白家族的孔蛋白。它有助于HIV-1 Rev介导的RNA输出，对于42 ℃下热休克RNA的输出也很重要。我们在这里表明，Rip 1 p是必不可少的热休克RNA的出口，这一功能是由Rip 1 p的独特的羧基末端没有实质性的贡献，从FG重复区。Rip 1 p和RNA输出介体Gle 1 p之间的遗传相互作用被描述，这支持Rip 1 p的羧基末端在poly（A）+ RNA输出中的作用。最后，Rip 1 p的这个结构域也有助于Rev介导的RNA输出。这些数据表明，Rip 1 p通过促进最佳孔功能促进不同类别底物的核输出。
The FG-repeat domain of the yeast Rip1 protein (Rip1p) was identified initially as a possible target for the nuclear export signal (NES) of the HIV-1 Rev protein in a yeast two-hybrid assay. Rip1p is inessential, associated with nuclear pore complexes, and structurally related to the FG-nucleoporin family of pore proteins. It contributes to HIV-1 Rev-mediated RNA export and is also important for the export of heat shock RNAs at 42 degrees C. We show here that Rip1p is essential for the export of heat shock RNAs, and this function is fulfilled by the unique carboxyl terminus of Rip1p with no substantial contribution from the FG-repeat region. Genetic interactions between Rip1p and the RNA export mediator Gle1p are described, which support a role of the carboxyl terminus of Rip1p in poly(A)+ RNA export. Finally, this domain of Rip1p also contributes to Rev-mediated RNA export. The data suggest that Rip1p promotes the nuclear export of different classes of substrates by contributing to optimal pore function.
按功能克隆：识别其他生物体基因的酵母同源物的另一种方法。
DOI：
10.1073/pnas.87.17.6629
发表时间：
1990
影响因子：
11.1
作者：
Kranz,JE;Holm,C
通讯作者：
Holm,C
影响因子：
10.5
作者：
Saavedra, C;Tung, KS;Cole, CN
通讯作者：
Cole, CN