Membrane potential difference and intracellular cation concentrations in human placental trophoblast cells in culture
Membrane potential difference and intracellular cation concentrations in human placental trophoblast cells in culture
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DOI：
10.1113/jphysiol.1996.sp021333
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发表时间：
1996-05-01
影响因子：
5.5
通讯作者：
Sibley, CP
中科院分区：
文献类型：
--
作者：
Greenwood, SL;Clarson, LH;Sibley, CP
1.在滋养层细胞分化的细胞培养模型中，评估了Na+和K+的电化学梯度。测定绒毛膜癌细胞(JAR细胞系，96%为未分化滋养层细胞)以及分离自人胎盘的单核和多核(分化)滋养层细胞的膜电位差(E(M))、细胞内水分和Na+、K+含量。在JAR细胞中，E(M)从-57 mV下降到-48 mV和-40 mV，哇巴因(1 mM作用15分钟)使JAR细胞膜去极化15 mV，但不影响细胞滋养细胞膜电位。JAR细胞、单核细胞滋养层细胞和多核细胞滋养层细胞内K+浓度相似，但单核细胞滋养层细胞Na+浓度高于JAR细胞。哇巴因(3 mM作用15min)使单核细胞滋养层细胞含水量略有增加(4.5%)，但K+浓度降低(接近30%)，Na+浓度升高(接近125%)。所有细胞的K+平衡电位E(K)均大于E(M)，JAR细胞的E(K)和E(M)之差(-25 mV)小于单核和多核细胞滋养层细胞的E(K)和E(M)之差(分别为-33和-43 mV)。滋养层细胞的Na+平衡电位(E(Na))为正，JAR、单核和多核细胞滋养层细胞的E(Na)与E(M)之差分别为122、100和100 mV。这些结果表明，K+的电化学梯度受滋养层细胞分化阶段的影响。相反，在单核和多核细胞滋养层细胞中，Naf的电化学梯度是相似的。
1. The electrochemical gradients for Na+ and K+ were assessed in a cell culture model of trophoblast differentiation.2. Membrane potential difference (E(m)), intracellular water and Na+ and K+ contents were measured in choriocarcinoma cells (JAr cell line; 96% of which are undifferentiated trophoblast cells) and in mononucleate and multinucleate (differentiated) cytotrophoblast cells isolated from the human placenta at term.3. There was a significant fall in E(m) from -57 mV in JAr cells, to -48 and -40 mV in mono- and multinucleate cytotrophoblast cells, respectively Treatment with ouabain (1 mM for 15 min) depolarized the JAr cell membrane by 15 mV but did not affect cytotrophoblast cell membrane potential.4. Intracellular K+ concentration was similar in JAr, mono- and multinucleate cytotrophoblast cells but Na+ concentration was higher in mononucleate cytotrophoblast cells compared with JAr cells.5. Ouabain treatment (3 mM for 15 min) caused a small increase (4.5%) in cell water in mononucleate cytotrophoblast cells but lowered K+ (similar to 30%) and increased Na+ concentration (similar to 125 %) in all the trophoblast cells studied.6. The K+ equilibrium potential (E(K)) was more negative than E(m) in all cells and the difference between E(K) and E(m) was smaller in JAr cells (-25 mV) than in mono- and multinucleate cytotrophoblast cells (-33 and -43 mV, respectively).7. The Na+ equilibrium potential (E(Na)) was positive in the trophoblast cells and the difference between E(Na), and E(m) was 122, 100 and 100 mV in JAr, mono- and multinucleate cytotrophoblast cells, respectively.8. These results suggest that the electrochemical gradient for K+ is affected by the stage of trophoblast cell differentiation. In contrast, the electrochemical gradient for Naf is similar in mono- and multinucleate cytotrophoblast cells.