Determination of Malachite Green Residues in Fish Using a Highly Sensitive Electrochemiluminescence Method Combined with Molecularly Imprinted Solid Phase Extraction
Determination of Malachite Green Residues in Fish Using a Highly Sensitive Electrochemiluminescence Method Combined with Molecularly Imprinted Solid Phase Extraction
复制标题
高灵敏电化学发光结合分子印迹固相萃取法测定鱼类中孔雀石绿残留量
DOI：
10.1021/jf2008502
复制
发表时间：
2011-05-25
影响因子：
6.1
通讯作者：
Wang, Sui
中科院分区：
文献类型：
--
作者：
Guo, Zhiyong;Gai, Panpan;Wang, Sui
建立了电化学发光（ECL）抑制-分子印迹固相萃取（MISPE）联合测定鱼类中孔雀石绿（MG）残留量的方法。发现MG对鲁米诺的ECL信号有较强的抑制作用。在优化条件下，在20 ~ 5000 ppt的浓度范围内，淬灭ECL强度与MG浓度的对数呈良好的线性关系。方法检出限为6个百分点左右。合成分子印迹聚合物（MIPs）作为固相萃取（SPE）吸附剂，利用MISPE对MG进行选择性萃取和纯化。通过与2,3-二氯-5,6-二氰-1,4-苯醌（DDQ）的氧化反应，将白孔雀石绿（LMG）转化为MG，成功地将该方法应用于鱼中MG残留量的测定。并提出了MG对鲁米诺猝灭作用的可能机理。
An electrochemiluminescence (ECL) inhibition method combined with molecularly imprinted solid phase extraction (MISPE) was developed for quantitative determination of malachite green (MG) residues in fish. It was found that MG could strongly inhibit the ECL signal of luminol. Under the optimized conditions, the quenched ECL intensity versus the logarithm of the concentration of MG was in good linear relationship over a concentration range from 20 to 5000 ppt. The method detection limit was found to be about 6 ppt. Molecularly imprinted polymers (MIPs) were synthesized as solid phase extraction (SPE) sorbents, and MISPE was used for the selective extraction and purification of MG. By carrying out the oxidation reaction with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ), which could convert leucomalachite green (LMG) into MG, this method was successfully applied to determine MG residues in fish. A possible mechanism for the quenching effects of MG on luminol was also proposed.