Expression of genes coding for selected amino acid transporters in small intestine, liver, and skeletal muscle of pigs fed excess branched-chain amino acids
Expression of genes coding for selected amino acid transporters in small intestine, liver, and skeletal muscle of pigs fed excess branched-chain amino acids
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DOI：
10.4238/2015.august.19.11
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发表时间：
2015-01-01
影响因子：
0.4
通讯作者：
Morales, A.
中科院分区：
文献类型：
--
作者：
Cervantes, M.;Arce, N.;Morales, A.
过量的Leu可能通过减少猪体内Ile和瓦尔氨基酸（AA）的吸收来减少猪体内Ile和Val的可用性。采用24头杂交猪，研究了过量Leu单独或与过量Ile和瓦尔共同作用对小肠、肝脏和骨骼肌中阳离子（B（0）、（+）AT和CAT 1）和中性（B（0）AT 1）AA转运蛋白表达的影响。饮食治疗包括：1）基础日粮; 2）基础加0. 43% L-Leu（过量Leu）; 3）基础加0.43%Leu、0. 20% L-Ile和0.25%L-瓦尔（过量Leu、Ile和瓦尔）。基础日粮含有0.90%标准化回肠可消化赖氨酸，以及结晶L-Lys、L-Thr、DL-Met、L-Trp、L-Leu、L-Ile、L-His和L-Val。与基础日粮相比，日粮2和3含有多52%的Leu，日粮3含有多42%的Ile和瓦尔。过量的Leu或过量的Leu、Ile和瓦尔降低空肠中B（0），（+）AT的表达（P < 0.05），但对回肠和肝脏无影响。过量Leu使CAT 1在回肠的表达增加，但在肝脏的表达减少（P < 0.05）。过量的Leu、Ile和瓦尔可增加（P < 0.05）B（0）AT 1在空肠中的表达，并有增加（P = 0.10）回肠中AT 1表达的趋势。一般而言，空肠中B（0），（+）AT表达高于回肠，但CAT 1表达低于回肠; B（0）AT 1在空肠和回肠中表达相似。过量的Leu或任何支链AA影响AA转运蛋白的表达，这可能会影响动物生长对AA的吸收和可用性。
Excess Leu reduces the availability of Ile and Val in pigs likely by reducing absorption of the latter amino acids (AA). Twenty-four crossbred pigs were used to evaluate the effect of excess Leu alone or with surplus Ile and Val on the expression of cationic (b(0),(+)AT and CAT1) and neutral (B(0)AT1) AA transporters in the small intestine, liver, and skeletal muscle. Dietary treatments included: 1) basal diet; 2) basal plus 0.43% L-Leu (excess Leu); 3) basal plus 0.43% Leu, 0.20% L-Ile, and 0.25% L-Val (excess Leu, Ile, and Val). The basal diet contained 0.90% standardized ileal digestible Lys, as well as crystalline L-Lys, L-Thr, DL-Met, L-Trp, L-Leu, L-Ile, L-His, and L-Val. Diets 2 and 3 contained 52% more Leu and diet 3 contained 42% more Ile and Val compared with the basal diet. Excess Leu or excess Leu, Ile, and Val reduced b(0),(+)AT expression in the jejunum (P < 0.05) but had no effect in the ileum and liver. Excess Leu increased CAT1 expression in the ileum but reduced expression in the liver (P < 0.05). Excess Leu, Ile, and Val increased (P < 0.05) B(0)AT1 expression in the jejunum and tended to increase (P = 0.10) expression in the ileum. In general, b(0),(+)AT expression was higher but CAT1 expression was lower in the jejunum than in the ileum; B(0)AT1 was similarly expressed in the jejunum and ileum. Excess Leu or any branched-chain AA affects AA transporter expression, which may affect the absorption and availability of AA for animal growth.