The Alcohol and Epoxy Alcohol of Zingiberene, Produced in Trichomes of Wild Tomato, Are More Repellent to Spider Mites Than Zingiberene
The Alcohol and Epoxy Alcohol of Zingiberene, Produced in Trichomes of Wild Tomato, Are More Repellent to Spider Mites Than Zingiberene
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DOI：
10.3389/fpls.2020.00035
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发表时间：
2020-02-21
影响因子：
5.6
通讯作者：
Snyder, John C.
中科院分区：
文献类型：
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作者：
Dawood, Mohammad H.;Snyder, John C.
野生番茄品种的毛状分泌物中存在的化感物质在介导与节肢动物的相互作用中起主要作用，通常通过抗生素和抗异种作用赋予高水平的抗性。许多野生番茄的亲缘种，habrochaites (S.h)，对节肢动物具有高水平的抗性。单环倍半萜类化合物7-epi-紫姜烯（zingiberene）是一种主要的防御成分，存在于某些紫姜属植物的毛状分泌物中。我们利用紫姜属植物LA2329作为供体，设计了将紫姜烯渗入栽培番茄的育种计划。然而，在我们的LA2329群体中，毛分泌物的组成是分离的，一些个体在其分泌物中主要产生7-epi-zingiberene，而其他个体在其毛分泌物中产生另外两种未知的化合物。为了研究这些化合物是否也可能对节肢动物抗性起作用，我们收集了在温室条件下生长的S.h LA2329植株的毛状分泌物，然后用硅胶柱层析法分离了主要化合物，并测试了它们对两种斑蜘蛛螨（TSSM）和荨叶螨（Tetranychus urticae）的驱避能力。利用气相色谱/质谱法分离鉴定化感物质。结果表明，7-外延紫姜烯、9-羟基紫姜烯和9-羟基10,11-环氧紫姜烯三个色谱峰的存在是主要的。桥式生物实验结果表明，9-羟基紫姜烯和9-羟基10,11-环氧紫姜烯的驱避活性均显著高于7-外延紫姜烯。这些结果支持了不同植物化感物质的驱避程度可能存在差异的观点，也强调了将9-羟基紫姜烯和9-羟基10,11-环氧紫姜烯渗入栽培番茄以增强其对节肢动物抗性的潜在价值。
Allelochemicals that are present in trichome secretions of wild tomato species play a major role in mediating interactions with arthropods, often conferring a high level of resistance via antibiosis and antixenosis. Many accessions of the wild tomato relative, Solanum habrochaites (S.h), possess high levels of resistance to arthropods. The monocyclic sesquiterpene hydrocarbon, 7-epi-zingiberene, is a major defensive component found in trichome secretions of certain accessions of S.h. We have used LA2329, an S.h. accession, as a donor in a breeding program designed to introgress zingiberene into cultivated tomato. However, the composition of trichome secretions in our population of LA2329 is segregating, with some individuals producing mainly 7-epi-zingiberene in their secretions while others producing two additional, unidentified compounds in their trichome secretions. To investigate if these other compounds may also contribute to arthropod resistance, trichome secretions were collected from plants of S.h LA2329 grown under greenhouse conditions and then major compounds were isolated by silica gel column chromatography and tested for their ability to repel two spotted-spider mite (TSSM), Tetranychus urticae. Isolation and identification of allelochemicals were aided by use of gas chromatography/mass spectroscopy. The results revealed the presence of three predominate chromatographic peaks: 7-epi-zingiberene, 9-hydroxy zingiberene, and 9-hydroxy,10,11-epoxy-zingiberene. Results of testing isolated compounds for repellency to TSSM using bridge bioassays revealed that the repellent activities of 9-hydroxy zingiberene and 9-hydroxy,10,11-epoxy-zingiberene were each significantly higher than that for 7-epi-zingiberene. These results support the idea that the degree of repellency may differ among plant allelochemicals and also emphasize the potential value of introgressing the presence of 9-hydroxy zingiberene and 9-hydroxy,10,11-epoxy-zingiberene into cultivated tomato to enhance its arthropod resistance.