Sea cucumber peptides inhibit the malignancy of NSCLC by regulating miR-378a-5p targeted TUSC2
Sea cucumber peptides inhibit the malignancy of NSCLC by regulating miR-378a-5p targeted TUSC2
复制标题
海参肽通过调控miR-378a-5p靶向TUSC2抑制NSCLC恶性肿瘤
DOI：
10.1039/d1fo02267a
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发表时间：
2021-11-17
期刊：
影响因子：
6.1
通讯作者：
Zhang, Min
中科院分区：
文献类型：
--
作者：
Mao, Jing;Zhang, Zhuchi;Zhang, Min
肺癌是世界范围内死亡率较高的常见癌症，其中非小细胞肺癌（NSCLC）占绝大多数。非小细胞肺癌的临床治疗效果并不理想。本研究旨在探讨海参肽（SCP）对非小细胞肺癌（NSCLC）的抑制作用及其机制。结果表明，SCP能有效抑制A549细胞的增殖、迁移和侵袭。此外，SCP还能抑制肺癌小鼠胸腔积液的形成和肿瘤生长，减轻肝肾损伤，提高IL-2、IL-12水平，降低IL-6、TNF-α水平，延长小鼠生存时间。小鼠肿瘤的microRNA测序和免疫组化结果显示，miR-378 a-5 p靶向的肿瘤抑制基因TUSC 2参与SCP抑制肿瘤生长的作用。本研究为SCP进一步开发为抗肿瘤营养补充剂提供了实验依据，为探索食源性活性肽抗肿瘤应用的分子机制提供了新思路。
Lung cancer is a common cancer with high mortality worldwide, and non-small cell lung cancer (NSCLC) accounts for the majority. The clinical treatment effect of NSCLC is not ideal. The aim of this study was to investigate the inhibitory effect of sea cucumber peptide (SCP) on NSCLC and its mechanism. The results showed that SCP could effectively inhibit the proliferation, migration and invasion of A549 cells. In addition, SCP can also inhibit the formation of pleural effusion and tumor growth in lung cancer mice, reduce liver and kidney injury, increase the levels of IL-2 and IL-12, decrease the levels of IL-6 and TNF-alpha, and prolong the survival time of mice. The microRNA sequencing and immunohistochemistry of mouse tumors showed that the tumor suppressor gene TUSC2 targeted by miR-378a-5p was involved in the inhibition of tumor growth by SCP. This study provides an experimental basis for the further development of SCP as an anti-tumor nutritional supplement, and provides a new idea for exploring the molecular mechanism of food derived active peptides in anti-tumor applications.