Chromosome spatial clustering inferred from radiogenic aberrations
Chromosome spatial clustering inferred from radiogenic aberrations
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DOI：
10.1080/09553000410001723857
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发表时间：
2004-07-01
影响因子：
2.6
通讯作者：
Hlatky, L
中科院分区：
文献类型：
--
作者：
Arsuaga, J;Greulich-Bode, KM;Hlatky, L
目的：分析低线性能量转移 (LET) 辐射引起的染色体畸变，以表征人类淋巴细胞中 22 个人类常染色体之间的系统空间聚类，并比较它们在交换中的相对参与情况。材料和方法：多色荧光原位杂交 (mFISH) 数据集，指定人类外周血中期的颜色连接 体外暴露于低 LET 辐射 72 小时后，对淋巴细胞进行单独分析并结合之前发表的结果。蒙特卡洛计算机模拟和数学建模指导数据分析。结果和结论：畸变数据的统计测试证实了两个染色体簇 {1, 16, 17, 19, 22} 和 {13, 14, 15, 21, 22}，其成员之间的平均距离比随机预测的更接近。先前报道第一组位于间期核中心附近，主要由富含基因的染色体形成，而第二组包括核仁染色体。结果表明染色体定位和转录之间可能存在相互作用。文献中提出的许多其他簇尚未得到证实，并且存在相当大的染色体-染色体并置的随机性。此外，与之前的结果一致，我们发现染色体参与交换大约与 DNA 含量的三分之二次方成正比。
Purpose: Analysing chromosome aberrations induced by low linear energy transfer (LET) radiation in order to characterize systematic spatial clustering among the 22 human autosomes in human lymphocytes and to compare their relative participation in interchanges.Materials and methods: A multicolour fluorescence in situ hybridization (mFISH) data set, specifying colour junctions in metaphases of human peripheral blood lymphocytes 72 h after in vitro exposure to low LET radiation, was analysed separately and in combination with previously published results. Monte Carlo computer simulations and mathematical modelling guided data analysis.Results and conclusions: Statistical tests on aberration data confirmed two clusters of chromosomes, {1, 16, 17, 19, 22} and {13, 14, 15, 21, 22}, as having their members being on average closer to each other than randomness would predict. The first set has been reported previously to be near the centre of the interphase nucleus and to be formed mainly by gene-rich chromosomes, while the second set comprises the nucleolus chromosomes. The results suggest a possible interplay between chromosome positioning and transcription. A number of other clusters suggested in the literature were not confirmed and considerable randomness of chromosome-chromosome juxtapositions was present. In addition, and consistent with previous results, it was found that chromosome participation in interchanges is approximately proportional to the two-thirds power of the DNA content.