Identification and real-time imagine of a myc-expressing neutrophil population involved in inflammation and mycobacterial granuloma formation in zebrafish
Identification and real-time imagine of a myc-expressing neutrophil population involved in inflammation and mycobacterial granuloma formation in zebrafish
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DOI：
10.1016/j.dci.2007.04.003
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发表时间：
2008-01-01
影响因子：
2.9
通讯作者：
Spaink, Herman P.
中科院分区：
文献类型：
--
作者：
Meijer, Annemarie H.;van der Sar, Astrid M.;Spaink, Herman P.
通过增强子陷阱筛选，我们确定了一个转基因斑马鱼线显示白细胞特异性YFP表达在胚胎晚期和早期幼虫发育。它的增强子检测插入被定位在myc原癌基因家族的一个新成员附近，该家族编码已知对调节人类骨髓生成很重要的转录因子。斑马鱼myc家族的特征表明，只有这种特定的myc基因在白细胞中强烈表达。为了确定myc/YFP表达细胞类型，我们重新检查所描述的骨髓标记物的特异性，通过多重荧光原位杂交，显示lcp 1可以被认为是一个一般的白细胞标记物，csf 1 r作为巨噬细胞特异性标记物，和mpx和lyz作为嗜中性粒细胞特异性标记物。随后的共定位分析将YFP阳性细胞定义为中性粒细胞群体的子集。使用实时共聚焦成像，我们证明了这些细胞迁移到炎症部位，并参与对感染的先天免疫反应，包括海分枝杆菌诱导的肉芽肿形成。(C)2007爱思唯尔有限公司保留所有权利。
By enhancer trap screening we identified a transgenic zebrafish line showing leukocyte-specific YFP expression during late embryo and early larval development. Its enhancer detection insertion was mapped near a novel member of the myc proto-oncogene family, encoding transcription factors known to be important for regulating human myelopoiesis. Characterization of the zebrafish myc family showed that only this particular myc gene is strongly expressed in leukocytes. To identify the myc/YFP-expressing cell type, we reexamined specificity of described myeloid markers by multiplex fluorescent in situ hybridization, showing that lcp1 can be considered as a general leukocyte marker, csf1r as a macrophage-specific marker, and mpx and lyz as neutrophil-specific markers. Subsequent colocalization analysis defined the YFP-positive cells as a subset of the neutrophil population. Using real-time confocal imaging we demonstrate that these cells migrate to sites of inflammation and are involved in innate immune responses towards infections, including Mycobacterium marinum-induced granuloma formation. (C) 2007 Elsevier Ltd. All rights reserved.