Renal Denervation and Celiac Ganglionectomy Decrease Mean Arterial Pressure Similarly in Genetically Hypertensive Schlager (BPH/2J) Mice.
Renal Denervation and Celiac Ganglionectomy Decrease Mean Arterial Pressure Similarly in Genetically Hypertensive Schlager (BPH/2J) Mice.
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去肾神经和腹腔神经节切除术降低遗传性高血压Schlager(BPH/2J)小鼠的平均动脉压的作用类似。
DOI：
10.1161/hypertensionaha.119.14069
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Osborn JW
中科院分区：
文献类型：
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作者：
Asirvatham-Jeyaraj N;Gauthier MM;Banek CT;Ramesh A;Garver H;Fink GD;Osborn JW
补充数字内容可在文本中找到。去肾神经支配（RDNX）降低顽固性高血压患者的平均动脉压（MAP）。腹腔神经节切除术（CGX）的效果研究较少，CGX是一种切除支配内脏血管床的神经的手术。我们假设RDNX和CGX都可以通过降低交感神经张力来降低遗传性高血压Schlager（BPH/2 J）小鼠的MAP。将遥测仪植入小鼠的股动脉中以监测RDNX（n=5）、CGX（n=6）或SHAM（n=6）之前和之后的MAP。术后14天记录MAP、收缩压、舒张压和心率。在对照组第3天和术后第10天测量MAP对六甲双铵（10 mg/kg，IP）的反应，作为整体神经源性升压活性的测量。通过测量组织去甲肾上腺素来评估去神经支配的效果。手术治疗前3组的对照MAP相似（≤ 130 mm Hg）。术后第14天，RDNX组（−11±2 mm Hg）和CGX组（−11±1 mm Hg）的MAP显著低于去神经前的值。而在SHAM小鼠（−5±3 mm Hg）中情况并非如此。术后第10天，RDNX组对六甲双铵的降压反应（−10±5 mm Hg）显著小于基线对照组（−25±10 mm Hg）。在SHAM（第10天; −28±5 mm Hg）或CGX（第10天; −34±7 mm Hg）组的小鼠中，情况并非如此。总之，肾神经和内脏神经都有助于BPH/2 J小鼠的高血压，但可能通过不同的机制。
Supplemental Digital Content is available in the text. Renal denervation (RDNX) lowers mean arterial pressure (MAP) in patients with resistant hypertension. Less well studied is the effect of celiac ganglionectomy (CGX), a procedure which involves the removal of the nerves innervating the splanchnic vascular bed. We hypothesized that RDNX and CGX would both lower MAP in genetically hypertensive Schlager (BPH/2J) mice through a reduction in sympathetic tone. Telemeters were implanted into the femoral artery in mice to monitor MAP before and after RDNX (n=5), CGX (n=6), or SHAM (n=6). MAP, systolic blood pressure, diastolic blood pressure, and heart rate were recorded for 14 days postoperatively. The MAP response to hexamethonium (10 mg/kg, IP) was measured on control day 3 and postoperative day 10 as a measure of global neurogenic pressor activity. The efficacy of denervation was assessed by measurement of tissue norepinephrine. Control MAP was similar among the 3 groups before surgical treatments (≈130 mm Hg). On postoperative day 14, MAP was significantly lower in RDNX (−11±2 mm Hg) and CGX (−11±1 mm Hg) groups compared with their predenervation values. This was not the case in SHAM mice (−5±3 mm Hg). The depressor response to hexamethonium in the RDNX group was significantly smaller on postoperative day 10 (−10±5 mm Hg) compared with baseline control (−25±10 mm Hg). This was not the case in mice in the SHAM (day 10; −28±5 mm Hg) or CGX (day 10; −34±7 mm Hg) group. In conclusion, both renal and splanchnic nerves contribute to hypertension in BPH/2J mice, but likely through different mechanisms.