Si Nanowires Forest-Based On-Chip Biomolecular Filtering, Separation and Preconcentration Devices: Nanowires Do it All
Si Nanowires Forest-Based On-Chip Biomolecular Filtering, Separation and Preconcentration Devices: Nanowires Do it All
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DOI：
10.1021/nl3021889
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发表时间：
2012-09-01
期刊：
影响因子：
10.8
通讯作者：
Patolsky, Fernando
中科院分区：
文献类型：
--
作者：
Krivitsky, Vadim;Hsiung, Lo-Chang;Patolsky, Fernando
有效的生物分子分离和纯化技术的发展在现代基因组学、蛋白质组学和生物传感领域中至关重要，这主要是由于大多数生物样品是高度多样性和复杂性的混合物。大多数现有技术缺乏从复杂生物样品中快速和选择性地分离和浓缩特定目标蛋白的能力，并且难以与芯片上实验室传感装置集成。在这里，我们展示了一个芯片上的全硅纳米线过滤，选择性分离，分离和预浓缩平台的发展，直接分析全血和其他复杂的生物样品。所需的蛋白质分析物从原始生物样品的分离首先使用抗体改性的粗糙度控制的SiNW（硅纳米线）森林的超大结合表面积，然后通过目标蛋白质在受控的液体介质中的释放，并随后通过超灵敏的SiNW基FET阵列在同一芯片平台上制造的检测。重要的是，这是首次展示用于在单个芯片上对血液样品进行整个直接分析的全NW装置，能够选择性地收集和分离特定的低丰度蛋白质，同时容易地去除不需要的血液成分（蛋白质、细胞）并实现分离效果，而不需要耗时的离心步骤、使用层析柱或亲和柱。此外，我们已经证明了我们的纳米线森林为基础的分离设备的使用，集成在一个单一的平台与下游SiNW为基础的传感器阵列，用于实时超灵敏检测蛋白质生物标志物直接从血液样本。整个超灵敏的蛋白质免标记分析过程实际上可以在不到10分钟的时间内完成。
The development of efficient biomolecular separation and purification techniques is of critical importance in modern genomics, proteomics, and biosensing areas, primarily due to the fact that most biosamples are mixtures of high diversity and complexity. Most of existent techniques lack the capability to rapidly and selectively separate and concentrate specific target proteins from a complex biosample, and are difficult to integrate with lab-on-a-chip sensing devices. Here, we demonstrate the development of an on-chip all-SiNW filtering, selective separation, desalting, and preconcentration platform for the direct analysis of whole blood and other complex biosamples. The separation of required protein analytes from raw biosamples is first performed using a antibody-modified roughness-controlled SiNWs (silicon nanowires) forest of ultralarge binding surface area, followed by the release of target proteins in a controlled liquid media, and their subsequent detection by supersensitive SiNW-based FETs arrays fabricated on the same chip platform. Importantly, this is the first demonstration of an all-NWs device for the whole direct analysis of blood samples on a single chip, able to selectively collect and separate specific low abundant proteins, while easily removing unwanted blood components (proteins, cells) and achieving desalting effects, without the requirement of time-consuming centrifugation steps, the use of desalting or affinity columns. Futhermore, we have demonstrated the use of our nanowire forest-based separation device, integrated in a single platform with downstream SiNW-based sensors arrays, for the real-time ultrasensitive detection of protein biomarkers directly from blood samples. The whole ultrasensitive protein label-free analysis process can be practically performed in less than 10 min.