Cyclic di-GMP differentially tunes a bacterial flagellar motor through a novel class of CheY-like regulators.
Cyclic di-GMP differentially tunes a bacterial flagellar motor through a novel class of CheY-like regulators.
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DOI：
10.7554/elife.28842
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发表时间：
2017-11-01
期刊：
影响因子：
7.7
通讯作者：
Jenal U
中科院分区：
文献类型：
--
作者：
Nesper J;Hug I;Kato S;Hee CS;Habazettl JM;Manfredi P;Grzesiek S;Schirmer T;Emonet T;Jenal U
鞭毛马达是一个复杂的旋转机器，促进运动和信号转导。由于其在细菌行为中的重要作用，其组装和活性受到严格调控。例如，趋化性依赖于通过马达开关蛋白CheY的磷酸化将化学信息耦合到马达的旋转偏置的感觉途径。使用化学蛋白质组学的方法，我们确定了一个新的家庭的Chey样（Cle）蛋白在新月柄杆菌，调整鞭毛活性响应结合的第二信使c-di-GMP的C-末端延伸。在它们的c-di-GMP结合构象中，Cle蛋白与鞭毛开关相互作用以控制运动活性。我们发现，个别Cle蛋白已通过离散的细胞功能，通过干扰趋化性和促进运动细胞的快速表面附着。这项研究拓宽了细菌马达的调节多样性，并揭示了将马达活动与机械线索和细菌表面适应联系起来的机制。
The flagellar motor is a sophisticated rotary machine facilitating locomotion and signal transduction. Owing to its important role in bacterial behavior, its assembly and activity are tightly regulated. For example, chemotaxis relies on a sensory pathway coupling chemical information to rotational bias of the motor through phosphorylation of the motor switch protein CheY. Using a chemical proteomics approach, we identified a novel family of CheY-like (Cle) proteins in Caulobacter crescentus, which tune flagellar activity in response to binding of the second messenger c-di-GMP to a C-terminal extension. In their c-di-GMP bound conformation Cle proteins interact with the flagellar switch to control motor activity. We show that individual Cle proteins have adopted discrete cellular functions by interfering with chemotaxis and by promoting rapid surface attachment of motile cells. This study broadens the regulatory versatility of bacterial motors and unfolds mechanisms that tie motor activity to mechanical cues and bacterial surface adaptation.