Loss of heterozygosity on the short arm of chromosome 3 in renal cancer
Loss of heterozygosity on the short arm of chromosome 3 in renal cancer
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DOI：
10.1159/000012149
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发表时间：
2000-01-01
期刊：
影响因子：
3.5
通讯作者：
Resel, L
中科院分区：
文献类型：
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作者：
Maestro, ML;del Barco, V;Resel, L
3%的人类癌症是肾细胞癌(RCC)。该肿瘤最常见的染色体异常是3号染色体短臂上的杂合性缺失(LOH)，提示在VHL基因附近3p14-3p21之间可能存在一个或多个抑癌基因，在肾癌的发生发展中起相关作用。应用聚合酶链式反应技术对40例不同分期的肾癌患者的正常肾组织和肿瘤组织的DNA进行3p(3p14.1-14.3；3p21.2-21.3和3p25)三个微卫星的杂合性缺失分析。在所研究的肿瘤中，42.5%的肿瘤至少有一个基因位点存在杂合性缺失。30%的人只在一个座位上发生杂合性缺失，5%的人在两个座位上发生LOH，7.5%的人在三个座位上发生杂合性缺失。LOH仅发生在非乳头状肿瘤(p=0.03)。有趣的是，3p21上LOH均大于或等于25 mm(p=0.04；相对危险度1.76，可信区间：1.3~2.3)。版权所有(C)2000 S.Karger AG。巴塞尔。
3% of human cancers are renal cell carcinomas (RCC). The most common chromosome abnormality found in this tumor is loss of heterozygosity (LOH) on the short arm of chromosome 3, which suggests that there must be one or more tumor suppressor genes between 3p14 and 3p21 near the VHL gene which play a relevant role in renal cancer development. DNA from normal and tumor tissue from 40 patients at various stages of RCC was analyzed for LOH at three microsatellites mapped to 3p (3p14.1-14.3; 3p21.2-21.3 and 3p25) by polymerase chain reaction). 42.5% of the tumors studied showed LOH on at least one locus. 30% showed LOH on only one locus; 5% on two loci and 7.5% on the three loci tested. LOH occurred only on nonpapillary tumors (p = 0.03). Interestingly, all the tumors with LOH on 3p21 were greater than or equal to 25 mm (p = 0.04; relative risk 1.76, confidence interval: 1.3-2.3). Copyright (C) 2000 S. Karger AG. Basel.