Response of the metabolic and autophagy pathways in Mytilus under starvation
Response of the metabolic and autophagy pathways in Mytilus under starvation
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DOI：
10.3389/fmars.2022.1014336
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发表时间：
2022-10
期刊：
影响因子：
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通讯作者：
B. Xie;Chuan Chen;Xiao-lin Zhang;Siwen Li;Zhong-qi Gu;Ji Huang;Jianfang He;P. Qi;B. Guo;Z. Liao;Xiaojun Yan
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作者：
B. Xie;Chuan Chen;Xiao-lin Zhang;Siwen Li;Zhong-qi Gu;Ji Huang;Jianfang He;P. Qi;B. Guo;Z. Liao;Xiaojun Yan
贻贝广泛分布于我国东部沿海一带，是一种具有重要工业价值的双壳类贝类。饥饿是贻贝在生长过程中经常遇到的一种环境压力。然而，关于贻贝在饥饿条件下的生理生化反应及分子调控和适应机制的研究报道较少。为了阐明贻贝宿主对饥饿反应的分子机制，采用Illumina/HiSeq-2000深度测序平台，对饥饿9 d前后的贻贝鳃转录组进行分析，并对差异表达基因（DEG）进行研究。从6个样本库中总共获得42.137 GB的干净数据，每个样本的平均干净数据量为7.023 GB，并且组装了58，540个平均长度为780 bp的unigenes。通过对NR、Swiss-Prot、Pfam、String、GO和KEGG数据库进行评论来说明Unigenes。饥饿9天后，分别有2，188和2，672个基因被确定为显著上调或下调表达的基因。其中，4,860个基因与260条通路相关，包含重要的富集通路，如“代谢”、“PI 3 K-Akt信号通路”、“吞噬体”、“凋亡”和“溶酶体”。在内含物中，通过电子显微镜研究了自噬细胞，并进一步观察了饥饿前后鳃组织中自噬相关基因的表达。数据表明自噬体的量增加，并且发现atg 2、atg 6和atg 13显著上调。这些结果表明，贻贝可能通过自噬来科普饥饿后的损伤。通过我们的研究结果，我们有助于更深入地了解贻贝在环境胁迫下免疫防御的分子机制，这对贻贝育种和理解贻贝免疫系统的分子机制具有重要意义。
Being an industrially valuable species of bivalve, Mytilus is widespread in the area around the eastern coast of China. Starvation is an environmental stress that mussels often encounter in their growth. However, few report carried out on the physiological and biochemical response and molecular regulation and adaptation mechanism of mussels under starvation. To illustrate the molecular mechanism of hosts of Mytilus to starvation, the gill transcriptome was used for analysis before and after 9 days of starvation in Mytilus using the Illumina/HiSeq-2000 deep sequencing platform, accompanied by the study of differentially expressed genes (DEGs). In total, 42.137 GB of clean data were obtained from six sample libraries, the average amount of clean data of each sample is 7.023 GB, and 58,540 unigenes that average 780 bp in length were assembled. Unigenes were illustrated by commenting them against the NR, Swiss-Prot, Pfam, String, GO and KEGG databases. After 9 days of starvation, 2,188 and 2,672 genes were determined to be significantly up- or down-regulated expression genes, separately. Amongst, 4,860 genes were associated with 260 pathways, contains vital enrichment pathways, such as “Metabolic”, “PI3K-Akt signaling pathway”, “Phagosome”, “Apoptosis” and “Lysosome”. In inclusion, autophagy cells were investigated by an electron microscopy, alongside further observations of the expression of autophagy-related genes in gill tissue before and after starvation. The data indicates that the amount of autophagosomes increased and found that atg2, atg6 and atg13 were significantly up-regulated. These results indicated that Mytilus may use autophagy to cope with their damage after starvation. With our research results, we have contributed to a deeper understanding of the role of the molecular mechanisms of immune defence in Mytilus under environmental stress, which has given insights into mussel breeding and the understanding of the molecular mechanisms of Mytilus immune system.