Heterogeneity and degree of TIMP4, GATA4, SOX18, and EGFL7 gene promoter methylation in non-small cell lung cancer and surrounding tissues
Heterogeneity and degree of TIMP4, GATA4, SOX18, and EGFL7 gene promoter methylation in non-small cell lung cancer and surrounding tissues
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DOI：
10.1016/j.cancergen.2011.07.010
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发表时间：
2011-09-01
期刊：
影响因子：
1.9
通讯作者：
Sverdlov, Eugene
中科院分区：
文献类型：
--
作者：
Azhikina, Tatyana;Kozlova, Alena;Sverdlov, Eugene
我们使用甲基化敏感的高分辨率解链分析来评估非小细胞肺癌和周围明显正常组织和非癌肺组织中TIMP 4、GATA 4、SOX 18和EGFL 7基因启动子内CpG岛的甲基化。我们发现，在肿瘤和周围正常组织中启动子甲基化是异质性的。这与健康肺组织相反，健康肺组织中的启动子通常是非甲基化或低甲基化的，甲基化的异质性较低。肿瘤周围正常组织中甲基化的异质性增加可能表明在遗传和形态学变化之前表观遗传过程的早期开始，并且可以用作早期癌变事件的生物标志物。该分析是研究表观遗传异质性的一种简单而敏感的工具，可用于临床实践。
We used methylation-sensitive high resolution melting analysis to assess methylation of CpG islands within the promoters of the TIMP4, GATA4, SOX18, and EGFL7 genes in samples of non small cell lung cancer and surrounding apparently normal tissue and noncancerous lung tissues. We found that the promoter methylation was heterogeneous in both tumor and surrounding normal tissue. This is in contrast to healthy lung tissue, where the promoters were normally either non- or hypomethylated, and the heterogeneity of methylation was low. An increased heterogeneity of methylation in the normal tissues surrounding the tumor may suggest an early start of epigenetic processes preceding genetic and morphologic changes and can be used as a biomarker of early cancerization events. This analysis is an easy and sensitive tool for studying epigenetic heterogeneity and could be used in clinical practice.