ESTABLISHMENT OF COMPARTMENTS IN DEVELOPING LEG IMAGINAL DISKS OF DROSOPHILA-MELANOGASTER
ESTABLISHMENT OF COMPARTMENTS IN DEVELOPING LEG IMAGINAL DISKS OF DROSOPHILA-MELANOGASTER
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DOI：
10.1007/bf00848882
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发表时间：
1976-01-01
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影响因子：
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通讯作者：
STEINER, E
中科院分区：
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作者：
STEINER, E
用X射线照射M/M+胚胎和幼虫诱导有丝分裂重组，获得了M+/M+基因型克隆。由于这些细胞比周围的M/M+细胞生长得更快，它们可以填充成虫盘的隔室内的大面积。目前的研究主要集中在三个腿盘。在3 ± 5岁的年龄范围内用1000 R剂量诱导克隆。0.5 h至144 h。所有的克隆诱导晚于胚盘阶段绝对限制在前或后室的光盘。前室和后室之间的边界沿着整条腿呈直线延伸，并在远端将2个爪分开。前室的进一步细分是由2龄幼虫开始的克隆所指示的。在后室中未检测到平行细分。在3龄早期的辐照导致克隆，仅限于单一的纵向刚毛行和腿段。但未发现明确的隔室边界;特别是近端-远端分离似乎不存在。在胚盘期诱导的318个克隆中，有11个克隆从翅延伸到第二腿，或从背索延伸到第三腿。除了3个克隆，显然占据了前，以及后室的翅膀或腿，所有克隆仍然局限于前或后室。克隆体的左前腿和右前腿经常重叠在一起。未观察到节段间重叠。最早的间室边界出现在所有胸椎间盘，这表明区室化是所有椎间盘共同的基本过程。
Using X-irradiation of M/M+ embryos and larvae to induce mitotic recombination, clones of M+/M+ genotype were obtained. Since such cells grow faster than the surrounding M/M+-cells they can fill large areas within the compartments of an imaginal disc. The present studies concentrated mainly on the 3 leg discs. Clones were induced by doses of 1000 R at ages ranging from 3 .+-. 0.5 h after oviposition to 144 h. All clones induced later than the blastoderm stage were absolutely restricted to either the anterior or the posterior compartment of a disc. The border between the anterior and posterior compartment runs as a straight line along the entire leg and at the distal end separates the 2 claws. A further subdivision of the anterior compartment is indicated by clones initiated in the 2nd larval instar. A parallel subdivision could not be detected in the posterior compartment. Irradiation in the early 3rd instar led to clones which were restricted to single longitudinal bristle rows and leg segments. But no clear-cut compartment borders could be found; in particular a proximo-distal separation appears to be absent. Among the 318 clones induced at the blastoderm stage, 11 extended from the wing into the 2nd leg, or from the haltere into the 3rd leg. With the exception of 3 clones that apparently occupied the anterior as well as the posterior compartment of a wing or a leg, all clones remained confined to either the anterior or the posterior compartment. Frequently clones overlapped left and right forelegs. Intersegmental overlaps were not observed. The earliest compartment borders appeared in all thoracic discs, suggesting that compartmentalization was a fundamental process common to all discs.