Cysteine-Zn2+ complexes:: unique molecular switches for inducible nitric oxide synthase-derived NO
Cysteine-Zn2+ complexes:: unique molecular switches for inducible nitric oxide synthase-derived NO
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DOI：
10.1096/fj.01-0240hyp
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发表时间：
2001-11-01
期刊：
影响因子：
4.8
通讯作者：
Kröncke, KD
中科院分区：
文献类型：
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作者：
Kröncke, KD
低纳摩尔范围内的一氧化氮(NO)作为一种跨细胞信使分子，通过与可溶性鸟苷环化酶血红素部分结合，启动附近靶细胞的调节和生理反应。炎症过程中由诱导型一氧化氮合酶(INOS)合成的较高的NO浓度显示出额外的作用：NO可能与O(2)反应，生成像N(2)O(3)这样的氮氧化物，能够亚硝化硫醇。参与细胞不同功能的各种蛋白质都含有半胱氨酸-锌(2+)复合体。讨论了NO在含有半胱氨酸-锌(2+)结构域的不同蛋白质中的作用，并在转录、蛋白质折叠和蛋白降解过程中发挥了重要作用。提示诱导型一氧化氮合酶产生的NO作为信号分子靶向半胱氨酸-锌(2+)连接，从而使细胞对亚硝化应激做出反应。
Nitric oxide (NO) in the low nanomolar range acts as a transcellular messenger molecule to initiate regulatory and physiological responses in nearby target cells via binding to the soluble guanylate cyclase heme moiety. Higher NO concentrations, as synthesized by the inducible NO synthase (iNOS) during inflammatory processes, show additional effects: NO may react with O(2), yielding nitrogen oxides like N(2)O(3) that are able to nitrosate thiols. A variety of proteins involved in very different functions of the cell contain cysteine-Zn(2+) complexes. Effects of NO on different proteins containing cysteine-Zn(2+) domains and playing essential roles during transcription, protein folding, and proteolysis are discussed. It is suggested that iNOS-derived NO acts as a signal molecule targeting cysteine-Zn(2+) linkages, thus enabling cells to react toward nitrosative stress.