Elevated Levels of Monocyte Chemoattractant Protein-1 in the Aqueous Humor after Phacoemulsification
Elevated Levels of Monocyte Chemoattractant Protein-1 in the Aqueous Humor after Phacoemulsification
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DOI：
10.1167/iovs.12-10231
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发表时间：
2012-12-01
影响因子：
4.4
通讯作者：
Tanihara, Hidenobu
中科院分区：
文献类型：
--
作者：
Kawai, Motofumi;Inoue, Toshihiro;Tanihara, Hidenobu
目的。目的：探讨超声乳化对单核细胞化学引诱蛋白-1 （MCP-1）水平的影响，并鉴定其细胞来源。为了临床研究，我们收集了21例白内障行超声乳化术和人工晶状体植入术前后（术后17.0 +/- 4.0个月）的房水样本。采用多重免疫分析法测定水溶液中MCP-1的水平。在动物实验中，兔行超声乳化（+/- IOL）。分别从未手术眼和手术眼采集房水标本，进行免疫测定。采用逆转录聚合酶链反应和免疫组织化学方法对眼睛进行分析。在临床研究中，术后平均（+/- SD）水溶液MCP-1水平（1773.5 +/- 321.2 pg/mL）高于术前（796.9 +/- 211.3 pg/mL, P < 0.0001）。在动物实验中，术后30天眼的平均MCP-1水相水平（pg/mL）（207.1 +/- 62.9）高于未手术眼（31.2 +/- 12.5,P = 0.018）。人工晶状体植入术未影响MCP-1水平的变化。超声乳化术后，角膜、虹膜、睫状体、囊袋中MCP-1 mRNA表达升高。MCP-1 mRNA在荚膜袋中的表达从30天到90天增加，而在其他组织中没有。免疫组织化学研究显示超声乳化术后后囊膜细胞MCP-1免疫反应阳性。超声乳化术后，人和动物眼中MCP-1水平均升高。晶状体囊上增生的晶状体上皮细胞可能是超声乳化术后MCP-1产生时间延长的主要细胞来源。(http://www.umin.ac.jp/number, UMIN000005788。)（Invest Ophthalmol Vis Sci. 2012;53:7951-7960） DOI: 10.1167/iovs.12-10231
PURPOSE. To elucidate the impact of phacoemulsification on aqueous monocyte chemoattractant protein-1 (MCP-1) levels, and identify its cell origin.METHODS. For clinical study, aqueous humor samples were collected before and after surgery (17.0 +/- 4.0 months postoperatively) from 21 cataract cases that underwent phacoemulsification and intraocular lens (IOL) implantation. Aqueous MCP-1 levels were determined using a multiplex immunoassay. For animal experiments, rabbits underwent phacoemulsification (+/- IOL). Aqueous humor samples were collected from nonoperated eyes and operated eyes, and immunoassays were performed. Eyes were analyzed by reverse transcription-polymerase chain reaction and immunohistochemical studies.RESULTS. In the clinical study, mean (+/- SD) aqueous MCP-1 levels were higher postoperatively (1773.5 +/- 321.2 pg/mL) than preoperatively (796.9 +/- 211.3 pg/mL; P < 0.0001). In animal experiments, mean aqueous MCP-1 levels (pg/mL) were higher in postoperative eyes on day 30 (207.1 +/- 62.9) than in nonoperated eyes (31.2 +/- 12.5; P = 0.018). IOL implantation did not affect the changes in MCP-1 levels. After phacoemulsification, MCP-1 mRNA expression was increased in the cornea, iris, ciliary body, and capsular bag. Expression of MCP-1 mRNA in the capsular bag, but not the other tissues, increased from day 30 to 90. Immunohistochemical studies showed positive immunoreactivity for MCP-1 in cells of the posterior capsule after phacoemulsification.CONCLUSIONS. Aqueous MCP-1 levels were elevated in both human and animal eyes after phacoemulsification. Proliferated lens epithelial cells on the capsule might be the major cell origin for prolonged MCP-1 production after phacoemulsification. (http://www.umin.ac.jp/number, UMIN000005788.) (Invest Ophthalmol Vis Sci. 2012;53:7951-7960) DOI: 10.1167/iovs.12-10231