Heterochronic Control of AFF-1-Mediated Cell-to-Cell Fusion in C. elegans
Heterochronic Control of AFF-1-Mediated Cell-to-Cell Fusion in C. elegans
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DOI：
10.1007/978-94-007-0763-4_2
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发表时间：
2011-01-01
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影响因子：
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通讯作者：
Podbilewicz, Benjamin
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作者：
Friedlander-Shani, Lilach;Podbilewicz, Benjamin
在正常发育中，细胞融合对于器官形成和有性生殖至关重要。线虫秀丽隐杆线虫已成为研究细胞间融合机制和发育功能的优秀系统。在这篇综述中，我们重点关注细胞融合的异时调节。异时基因控制线虫特定发育事件的时间。第一个被发现的 microRNA 被发现是影响异时发育和细胞间融合的突变。此外，许多异时转录因子也控制线虫中的特定细胞融合事件。我们描述了表皮缝细胞细胞融合的异时调节的已知情况。融合剂 AFF-1 先前已被证明可介导外侧表皮接缝细胞的融合。在这里，我们提供了支持该模型的证据，其中 LIN-29（控制接缝细胞末端融合的异时性锌指转录因子）在接缝细胞融合之前刺激 AFF-1 表达。因此，异时基因 LIN-29 控制 AFF-1 介导的细胞间融合，作为表皮接缝细胞终末分化程序的一部分。
In normal development cell fusion is essential for organ formation and sexual reproduction. The nematode Caenorhabditis elegans has become an excellent system to study the mechanisms and developmental functions of cell-to-cell fusion. In this review we focus on the heterochronic regulation of cell fusion. Heterochronic genes control the timing of specific developmental events in C. elegans. The first microRNAs discovered were found as mutations that affect heterochronic development and cell-cell fusions. In addition numerous heterochronic transcription factors also control specific cell fusion events in C. elegans. We describe what is known about the heterochronic regulation of cell fusion of the epidermal seam cells. The fusogen AFF-1 was previously shown to mediate the fusion of the lateral epidermal seam cells. Here we provide evidence supporting the model in which LIN-29, the heterochronic Zinc-finger transcription factor that controls the terminal fusion of the seam cells, stimulates AFF-1 expression in the seam cells before they fuse. Therefore, the heterochronic gene LIN-29 controls AFF-1-mediated cell-cell fusion as part of the terminal differentiation program of the epidermal seam cells.