Optical properties of protein monolayers during assembly
Optical properties of protein monolayers during assembly
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DOI：
10.1063/1.476917
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发表时间：
1998-08-22
影响因子：
4.4
通讯作者：
Ramsden, JJ
中科院分区：
文献类型：
--
作者：
Guemouri, L;Ogier, J;Ramsden, JJ
通过让溶液中的蛋白质分子沉积在固/液界面上来构建蛋白质单层。使用光波导光模式光谱（OWLS）跟踪这些层的结构演变。使用均匀薄膜模型解释测量的有效折射率。对于相对刚性的球状蛋白（转铁蛋白），层的厚度已经在非常低的表面覆盖率下确定，并且折射率在沉积过程中增加。对于高度伸长且柔韧的蛋白质（纤连蛋白）来说，情况恰恰相反。农民的情况与经典的随机顺序吸附一致。后一个结果意味着在沉积过程中不断发生构象重排，产生致密的蛋白质膜。 (C) 1998 年美国物理研究所。 [S0021-9606(98)70132-1]
Protein monolayers were built up by allowing protein molecules in solution to deposit at the solid/liquid interface. The structural evolution of these layers was followed using optical waveguide lightmode spectroscopy (OWLS). The measured effective refractive indices were interpreted using the uniform thin film model. For a relatively rigid, spheroidal protein (transferrin), the thickness of the layer was already established at very low surface coverages, and the refractive index increased during deposition. For a highly elongated and flexible protein (fibronectin), the converse occurred. The farmer case is consistent with classical random sequential adsorption. The latter result implies constant conformational rearrangement during deposition producing a compact proteinaceous membrane. (C) 1998 American Institute of Physics. [S0021-9606(98)70132-1]