Development of Clustered Resistance Gene Analogs-Based Markers of Resistance to Phytophthora capsici in Chili Pepper
Development of Clustered Resistance Gene Analogs-Based Markers of Resistance to Phytophthora capsici in Chili Pepper
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DOI：
10.1155/2019/1093186
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发表时间：
2019-01-01
影响因子：
--
通讯作者：
Yeom, Seon-In
中科院分区：
文献类型：
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作者：
Kim, Nayoung;Kang, Won-Hee;Yeom, Seon-In
土传病原辣椒疫霉（Phytophthora capsici）对辣椒属植物造成严重危害。辣椒对疫病的抗性是由位于第5染色体上的多个微效数量性状基因座（QTL）和一个一致的主效QTL控制的。辣椒5号染色体上的分子标记已被开发出来，以确定主要的遗传贡献的阻力，但取得了很大的成功。在这项研究中，以前报道的分子标记被用来重新分析的主要QTL区域上的第5染色体（6.2 Mbp至139.2 Mbp）。在扩展的主效QTL区域中鉴定了候选抗性基因类似物（RGA），包括14个核苷酸结合位点富含亮氨酸的重复序列，3个受体样激酶和1个受体样蛋白。在分别对辣椒疫霉具有抗性和敏感性的两种辣椒属胚种之间进行候选RGA的序列比较。通过高分辨率解链分析，筛选出11个与辣椒疫病抗性主效QTL紧密连锁的新的RGA标记。其中，CaNB-5480表现出最高的共分离率为86.9%，并可以应用于基因型的微生物是不适合以前的标记。CaNB-5480、CaRP-5130和CaNB-5330 3个标记的组合提高了61份辣椒种质的基因分型准确率。这些结果可用于辣椒抗疫病种质资源的高通量筛选和抗病基因的进一步鉴定。
The soil-borne pathogen Phytophthora capsici causes severe destruction of Capsicum spp. Resistance in Capsicum against P. capsici is controlled by numerous minor quantitative trait loci (QTLs) and a consistent major QTL on chromosome 5. Molecular markers on Capsicum chromosome 5 have been developed to identify the predominant genetic contributor to resistance but have achieved little success. In this study, previously reported molecular markers were used to reanalyze the major QTL region on chromosome 5 (6.2 Mbp to 139.2 Mbp). Candidate resistance gene analogs (RGAs) were identified in the extended major QTL region including 14 nucleotide binding site leucine-rich repeats, 3 receptor-like kinases, and 1 receptor-like protein. Sequence comparison of the candidate RGAs was performed between two Capsicum germplasms that are resistant and susceptible, respectively, to P. capsici. 11 novel RGA-based markers were developed through high-resolution melting analysis which were closely linked to the major QTL for P. capsici resistance. Among the markers, CaNB-5480 showed the highest cosegregation rate at 86.9% and can be applied to genotyping of the germplasms that were not amenable by previous markers. With combination of three markers such as CaNB-5480, CaRP-5130 and CaNB-5330 increased genotyping accuracy for 61 Capsicum accessions. These could be useful to facilitate high-throughput germplasm screening and further characterize resistance genes against P. capsici in pepper.