Automated light-based mapping of motor cortex by photoactivation of channelrhodopsin-2 transgenic mice
Automated light-based mapping of motor cortex by photoactivation of channelrhodopsin-2 transgenic mice
复制标题
DOI：
10.1038/nmeth.1303
复制
发表时间：
2009-03-01
期刊：
影响因子：
48
通讯作者：
Murphy, Timothy H.
中科院分区：
文献类型：
--
作者：
Ayling, Oliver G. S.;Harrison, Thomas C.;Murphy, Timothy H.
传统上，绘制运动皮层图谱需要电极刺激大脑并确定运动输出通路。尽管有效，但基于电极的方法劳动强度大，可能对皮层造成损伤，并且可能产生脱靶效应。作为运动图谱绘制的一种替代方法，我们对在主要是第5层输出皮层神经元中表达光敏感离子通道视紫红质 - 2的转基因小鼠进行光刺激。我们报告称，使用载物台扫描激光系统在体内对这些神经元进行光刺激会在10 - 20毫秒内导致肌肉兴奋，这可以通过植入的肌电图电极或无创运动传感器记录下来。这种方法使我们能够以比以前的方法快得多的速度绘制出小鼠前肢和后肢运动皮层的高度可重复的自动化图谱。我们预计这种方法将有助于研究在技能训练或神经系统受损后运动图谱的位置和特性的变化。
Traditionally, mapping the motor cortex requires electrodes to stimulate the brain and define motor output pathways. Although effective, electrode-based methods are labor-intensive, potentially damaging to the cortex and can have off-target effects. As an alternative method of motor mapping, we photostimulated transgenic mice expressing the light-sensitive ion channel channelrhodopsin-2 in predominantly layer-5 output cortical neurons. We report that optical stimulation of these neurons in vivo using a stage scanning laser system resulted in muscle excitation within 10-20 ms, which can be recorded using implanted electromyogram electrodes or by a noninvasive motion sensor. This approach allowed us to make highly reproducible automated maps of the mouse forelimb and hindlimb motor cortex much faster than with previous methods. We anticipate that the approach will facilitate the study of changes in the location and properties of motor maps after skilled training or damage to the nervous system.