Expression of α-glucosidase during morphological differentiation in the basidiomycetous fungus Pholiota microspora
Expression of α-glucosidase during morphological differentiation in the basidiomycetous fungus Pholiota microspora
复制标题
担子菌小孢黄伞形态分化过程中α-葡萄糖苷酶的表达
DOI：
10.1002/jobm.201500752
复制
发表时间：
2016
影响因子：
3.1
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Zhu;G.;Hayashi;M.;Shimomura;N.;Yamaguchi;T. and Aimi;T.
从小孢伞（Pholiota microspora）中扩增α-葡萄糖苷酶基因，命名为PnGcs。PnGcs的开放阅读框架区（从ATG到终止密码子）为2937 bp，编码979个氨基酸的蛋白质，N末端含有20个氨基酸的信号肽。预测的氨基酸序列表明PnGcs是糖苷水解酶家族31蛋白。采用定量逆转录PCR技术研究了PnGcs在不同碳源的基本培养基中培养的菌丝体以及子实体发育不同时期组织中的表达。当P.小孢子菌在不同碳源的基本培养基中生长，PnGcs在麦芽糖诱导下表达量最高。在木屑培养基上培养时，子实体形成期PnGcs的表达量明显高于菌丝生长期，表明PnGcs与子实体的发育密切相关。
The α‐glucosidase gene fromPholiota microspora, designatedPnGcs, was amplified and characterized. The open reading frame region ofPnGcs, from ATG to the stop codon, is 2937 bp and encodes a protein of 979 amino acids with a signal peptide of 20 amino acids at the N‐terminus. The predicted amino acid sequence of PnGcs indicated that it is a glycoside hydrolase family 31 protein. Quantitative reverse transcription PCR was used to investigatePnGcsexpression in mycelia cultured in minimal medium containing various carbon sources, as well as in tissue during different stages of development of fruiting bodies. WhenP. microsporawas grown in minimal medium supplemented with different carbon sources,PnGcsexpression was highest when induced by maltose. During cultivation on sawdust medium,PnGcsexpression increased dramatically at the fruiting body formation stage compared with the mycelial growth stage, which implied thatPnGcsis closely associated with fruiting body development.