Influence of the penetration enhancer isopropyl myristate on stratum corneum lipid model membranes revealed by neutron diffraction and 2H NMR experiments
Influence of the penetration enhancer isopropyl myristate on stratum corneum lipid model membranes revealed by neutron diffraction and 2H NMR experiments
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DOI：
10.1016/j.bbamem.2017.01.029
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发表时间：
2017-05-01
影响因子：
3.4
通讯作者：
Neubert, Reinhard H. H.
中科院分区：
文献类型：
--
作者：
Eichner, Adina;Stahlberg, Soeren;Neubert, Reinhard H. H.
角质层（SC）提供天然皮肤的主要屏障特性。屏障功能归因于细胞间脂质，形成连续的多层膜。在这项研究中，SC脂质膜的模型比例富含氘代脂质，以研究结构和动力学性质的中子衍射和H-2固态NMR光谱。此外，研究了渗透增强剂肉豆蔻酸异丙酯（IPM）对含有合成衍生的甲基支化神经酰胺[EDS]、神经酰胺[AP]、二十二烷酸和胆固醇（23/10/33/33重量%）的公知SC脂质模型膜的结构的影响。IPM支持形成一个单一的短周期相（SPP），在此期间，我们首次确定了CER[AP]和CER[EOS]-br的分子结构。此外，通过额外的中子衍射研究以及通过H-2固态NMR光谱分析脂质系统的热致相行为，覆盖32 ° C（生理皮肤温度）、50 ° C和70 ° C的温度，随后冷却回到皮肤温度。这两种技术揭示了相变和滞后效应。在冷却过程中，布拉格峰对应的长周期相（LPP）出现。此外，H-2 NMR显示IPM分子在所有温度下都是同位素移动的。(C)2017爱思唯尔B. V.保留所有权利。
The stratum corneum (SC) provides the main barrier properties in native skin. The barrier function is attributed to the intercellular lipids, forming continuous multilamellar membranes. In this study, SC lipid membranes in model ratios were enriched with deuterated lipids in order to investigate structural and dynamical properties by neutron diffraction and H-2 solid-state NMR spectroscopy. Further, the effect of the penetration enhancer isopropyl myristate (IPM) on the structure of a well-known SC lipid model membrane containing synthetically derived methyl-branched ceramide [EDS], ceramide [AP], behenic acid and cholesterol (23/10/33/33 wt%) was investigated. IPM supported the formation of a single short-periodicity phase (SPP), in which we determined the molecular organization of CER[AP] and CER[EOS]-br for the first time. Furthermore, the thermotropic phase behavior of the lipid system was analyzed by additional neutron diffraction studies as well as by H-2 solid-state NMR spectroscopy, covering temperatures of 32 degrees C (physiological skin temperature), 50 degrees C, and 70 degrees C with a subsequent cooldown back to skin temperature. Both techniques revealed a phase transition and a hysteresis effect. During the cooldown, Bragg peaks corresponding to a long-periodicity phase (LPP) appeared. Additionally, H-2 NMR revealed that the IPM molecules are isotopic mobile at all temperatures. (C) 2017 Elsevier B.V. All rights reserved.