IDENTIFICATION OF PEPTIDE SEQUENCES THAT POTENTIALLY TRIGGER HLA-A2.1-RESTRICTED CYTOTOXIC T-LYMPHOCYTES
IDENTIFICATION OF PEPTIDE SEQUENCES THAT POTENTIALLY TRIGGER HLA-A2.1-RESTRICTED CYTOTOXIC T-LYMPHOCYTES
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DOI：
10.1002/eji.1830230603
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发表时间：
1993-06-01
影响因子：
5.4
通讯作者：
KAST, WM
中科院分区：
文献类型：
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作者：
NIJMAN, HW;HOUBIERS, JGA;KAST, WM
我们使用人加工缺陷细胞系174CEM.T2（T2）来鉴定人蛋白质的潜在细胞毒性T淋巴细胞（CTL）表位。外源添加的肽可以增加T2细胞的细胞表面上正确折叠的HLA-A2.1分子的数量，如使用小鼠单克隆抗体BB7.2（抗HLA-A2. 1）和异硫氰酸荧光素标记的山羊抗小鼠F（ab '）2抗体的免疫荧光测量所示。这些肽是根据最近描述的HLA-A2.1特异性基序的计算机评分来选择的。对流感基质蛋白的分析显示，35种高分肽中的15种上调T2细胞表面上HLA-A2.1分子的表达。计算机评分程序和基于免疫荧光的肽结合测定的组合允许快速检测潜在的CTL靶肽。
We used the human processing defective cell line 174CEM.T2 (T2) to identify potential cytotoxic T lymphocyte (CTL) epitopes of human proteins. Exogenously added peptides can increase the number of properly folded HLA-A2.1 molecules on the cell surface of T2 cells, as shown by immunofluorescence measurements using the mouse monoclonal antibody BB7.2 (anti-HLA-A2.1) and fluorescein isothiocyanate-labeled goat anti-mouse F(ab')2 antibody. The peptides were selected on the basis of a computer score derived from the recently described HLA-A2.1 specific motif. Analysis of the influenza matrix protein showed that 15 out of 35 high-scoring peptides up-regulate the expression of HLA-A2.1 molecules on the T2 cell surface. The combination of the computer scoring program and an immunofluorescence-based peptide binding assay allows rapid detection of potential CTL target peptides.