Polyaniline based nucleic acid sensor.
Polyaniline based nucleic acid sensor.
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DOI：
10.1021/jp711853q
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发表时间：
2008-03
期刊：
影响因子：
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通讯作者：
N. Prabhakar;K. Arora;Harpal Singh;B. Malhotra
中科院分区：
文献类型：
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作者：
N. Prabhakar;K. Arora;Harpal Singh;B. Malhotra
将针对病原体（结核分枝杆菌）的 20 个碱基长的 NH2 修饰 DNA 和 PNA 探针共价固定到聚苯胺 (PANI)/Au 电极上，使用亚甲蓝在 30 秒内检测与互补、一碱基错配和非互补靶标的核酸杂交。与DNA-PANI/Au电极(2.5×10(-18)M)相比，PNA-PANI/Au电极表现出改进的特异性(1000倍)和检测限(0.125×10(-18)M)。这些 PNA-PANI/Au 电极可用于在 1 分钟杂交时间内检测与 5 分钟超声处理的结核分枝杆菌基因组 DNA 中的互补序列的杂交。这些 DNA-PANI/Au 和 PNA-PANI/Au 电极分别可使用 6-7 次和 13-15 次。
Twenty-bases long NH2-modified DNA and PNA probes specific to a pathogen (Mycobacterium tuberculosis) were covalently immobilized onto a polyaniline (PANI)/Au electrode to detect nucleic acid hybridization with complementary, one-base mismatch and noncomplementary targets within 30 s using Methylene Blue. The PNA-PANI/Au electrode exhibits improved specificity (1000 times) and detection limit (0.125 x 10(-18) M) as compared to that of the DNA-PANI/Au electrode (2.5 x 10(-18) M). These PNA-PANI/Au electrodes can be utilized for detection of hybridization with the complementary sequence in 5 min sonicated M. tuberculosis genomic DNA within 1 min of hybridization time. These DNA-PANI/Au and PNA-PANI/Au electrodes can be used 6-7 and 13-15 times, respectively.