Diet Consisting of Balanced Yogurt, Fruit, and Vegetables Modifies the Gut Microbiota and Protects Mice against Nonalcoholic Fatty Liver Disease
Diet Consisting of Balanced Yogurt, Fruit, and Vegetables Modifies the Gut Microbiota and Protects Mice against Nonalcoholic Fatty Liver Disease
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DOI：
10.1002/mnfr.201900249
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发表时间：
2019-08-16
影响因子：
5.2
通讯作者：
Wang, Li-Shun
中科院分区：
文献类型：
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作者：
Kong, Chao-Yue;Li, Zhan-Ming;Wang, Li-Shun
背景热量限制（CR）是治疗非酒精性脂肪肝的有效方法。然而，CR方法的依从性相对较差。需要新的CR方法。方法和结果每周给予小鼠5天的高脂肪饮食（HFD）自由采食加2天的间歇性卡路里限制（ICR）饮食（50%卡路里限制），持续16周，所述间歇性卡路里限制（ICR）饮食由酸奶、水果和蔬菜组成。检查ICR饮食模型对小鼠脂肪肝的影响。与连续HFD喂养的小鼠相比，HFD+ICR喂养的小鼠具有较低的体重和肝脏脂肪变性，降低血脂和转氨酶水平，增加Cpt 1a的脂肪酸氧化基因，降低Ppar γ和Srebf-1c的肝脏脂质合成基因，以及改善胰岛素抵抗和降低炎症水平。此外，ICR逆转了HFD组的菌群失调，包括较低的Shannon多样性指数和较低的乳酸杆菌丰度。结论由酸奶、水果和蔬菜组成的ICR饮食可减轻HFD诱导的小鼠肝脂肪变性的发展。此外，HFD+ICR饮食与不同的粪便微生物群相关，这些微生物群往往与正常饮食对照更相似。
Background Calorie restriction (CR) is a therapeutically effective method for nonalcoholic fatty liver disease. However, the compliance of the CR method is relatively poor. New CR methods are needed. Methods and Results Each week, mice are given a 5-day high-fat diet (HFD) ad libitum plus 2 days of an intermittent calorie restriction (ICR) diet (50% calorie restriction) consisting of yogurt, fruit, and vegetables, for 16 weeks. The effect of the ICR diet model on the fatty liver of mice is examined. Compared with continuous HFD-fed mice, the mice feeding HFD+ICR have lower body weight and hepatic steatosis, reduced serum lipid and transaminase levels, increased fatty acid oxidation gene of Cpt1a, and decreased hepatic lipid synthesis gene of Ppar gamma and Srebf-1c, as well as improved insulin resistance and lower level of inflammation. Moreover, ICR reverses the dysbacteriosis in HFD group, including the lower Shannon diversity indexes and lower abundance of Lactobacillus. Conclusion An ICR diet consisting of yogurt, fruit, and vegetables attenuates the development of HFD-induced hepatic steatosis in mice. Furthermore, HFD+ICR diet is associated with a different fecal microbiota that tends to be more similar to normal diet controls.