Proteomic Analysis Reveals Novel Extracellular Virulence-Associated Proteins and Functions Regulated by the Diffusible Signal Factor (DSF) in Xanthomonas oryzae pv. oryzicola
Proteomic Analysis Reveals Novel Extracellular Virulence-Associated Proteins and Functions Regulated by the Diffusible Signal Factor (DSF) in Xanthomonas oryzae pv. oryzicola
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蛋白质组学分析揭示了米黄单胞菌 pv. 中受扩散信号因子 (DSF) 调节的新型细胞外毒力相关蛋白质和功能。
DOI：
10.1021/pr4001543
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发表时间：
2013-07-01
影响因子：
4.4
通讯作者：
Liu, Fengquan
中科院分区：
文献类型：
--
作者：
Qian, Guoliang;Zhou, Yijing;Liu, Fengquan
黄单胞菌烟草致病变种（Xanthomonaspluspv.）水稻细菌性条斑病的致病因子是由扩散信号因子（DSF）介导的。DSF介导的QS已被证明控制毒力和一组毒力相关的功能;然而，由DSF信号控制的胞外蛋白的表达谱和功能在很大程度上仍不清楚。在本研究中，33 DSF调节的胞外蛋白，其功能包括小蛋白介导的QS，氧化适应，大分子代谢，细胞结构，小分子的生物合成，中间代谢，细胞过程，蛋白质催化剂，和假设的功能，在Xoc的蛋白质组学鉴定。其中，15个蛋白编码基因被框内删除，其中4个，包括3个编码II型分泌系统（T2 SS）依赖性蛋白的基因和1个编码Ax 21（XA 21介导的免疫激活剂）样蛋白（一种新的小蛋白型QS信号）的基因被确定为Xoc中完全毒力所需。这四个基因的贡献，重要的毒力相关的功能，包括细菌定植，胞外多糖，细胞运动，生物膜的形成，和抗氧化能力，提出。据我们所知，我们的分析是第一个完整的列表DSF调节细胞外蛋白和功能的黄单胞菌属物种。我们的研究结果表明，DSF-type QS在T2 SS和Ax 21介导的QS的调节中起着关键作用，这揭示了DSF信号在黄单胞菌中的作用。
Quorum sensing (QS) in Xanthomonas oryzae pv. oryzicola (Xoc), the causal agent of bacterial leaf streak, is mediated by the diffusible signal factor (DSF). DSF-mediating QS has been shown to control virulence and a set of virulence-related functions; however, the expression profiles and functions of extracellular proteins controlled by DSF signal remain largely unclear. In the present study, 33 DSF-regulated extracellular proteins, whose functions include small-protein mediating QS, oxidative adaptation, macromolecule metabolism, cell structure, biosynthesis of small molecules, intermediary metabolism, cellular process, protein catabolism, and hypothetical function, were identified by proteomics in Xoc. Of these, 15 protein encoding genes were in-frame deleted, and 4 of them, including three genes encoding type II secretion system (T2SS)-dependent proteins and one gene encoding an Ax21 (activator of XA21-mediated immunity)-like protein (a novel small-protein type QS signal) were determined to be required for full virulence in Xoc. The contributions of these four genes to important virulence-associated functions, including bacterial colonization, extracellular polysaccharide, cell motility, biofilm formation, and antioxidative ability, are presented. To our knowledge, our analysis is the first complete list of DSF-regulated extracellular proteins and functions in a Xanthomonas species. Our results show that DSF-type QS played critical roles in regulation of T2SS and Ax21-mediating QS, which sheds light on the role of DSF signaling in Xanthomonas.