Colocalization of somatostatin, neuropeptide Y, neuronal nitric oxide synthase and NADPH-diaphorase in striatal interneurons in rats
Colocalization of somatostatin, neuropeptide Y, neuronal nitric oxide synthase and NADPH-diaphorase in striatal interneurons in rats
复制标题
DOI：
10.1016/0006-8993(96)00801-3
复制
发表时间：
1996-10-07
期刊：
影响因子：
2.9
通讯作者：
Reiner, A
中科院分区：
文献类型：
--
作者：
FigueredoCardenas, G;Morello, M;Reiner, A
神经肽生长抑素（SS）、神经肽Y（NPY）、神经元型一氧化氮合酶（nNOS）和NADPH黄递酶（NADPHd）的酶活性广泛共定位于纹状体中间神经元中，这导致了广泛的趋势，即操作性地将所有四种物质视为完全共定位于单一类别的纹状体中间神经元内，我们已经探讨了这一假设的有效性，在大鼠纹状体使用多重标记的方法。用常规落射荧光显微镜检查SS、NPY和nNOS三重标记、SS和nNOS双标记或SS和NPY双标记的组织。在SS和nNOS双标记的组织中，将SS和nNOS标记的共聚焦激光扫描显微镜（CLSM）图像与随后的NADPHd标记进行比较。我们发现，SS，NPY和nNOS共同发生广泛，但也观察到一个中等丰富的人口含有SS和nNOS，但没有NPY的神经元，因为是小人口的SS只有和nNOS只有神经元。约80%的SS +神经元含有NPY，没有NPY神经元缺乏SS或nNOS。在大鼠纹状体内所有含nNOS的神经元均含有NADPHd。结合我们的各种定量观察，我们发现，在那些纹状体神经元含有任何组合的SS，NPY，nNOS和NADPH d的大鼠，约73%的含有所有四个，16%含有SS，nNOS和NADPH d，5%只含有SS，和6%只含有nNOS和NADPH d。这些结果表明，虽然大鼠中存在大量的纹状体神经元，其中SS、NPY、nNOS和NADPHd共存，但可能存在较少的缺乏NPY的纹状体神经元，其中SS或nNOS/NADPHd单独或一起存在。
The neuropeptides somatostatin (SS), neuropeptide Y (NPY), the enzyme neuronal nitric oxide synthase (nNOS) and enzymatic activity for NADPH diaphorase (NADPHd) are extensively colocalized in striatal interneurons, which has led to the widespread tendency to operationally treat al four substances as being completely colocalized within a single class of striatal interneurons, We have explored the validity of this assumption in rat striatum using multiple-labeling methods. Conventional epi-illumination fluorescence microscopy was used to examine tissue triple labeled for SS, NPY and nNOS, or double-labeled for SS and nNOS or for SS and NPY, In tissue double-labeled for SS and nNOS, confocal laser scanning microscopy (CLSM) images of SS and nNOS labeling were compared to subsequent NADPHd labeling. We found that SS, NPY and nNOS co-occurred extensively, but a moderately abundant population of neurons containing SS and nNOS but not NPY was also observed, as were small populations of SS only and nNOS only neurons. About 80% of SS + neurons contained NPY, and no NPY neurons were devoid of SS or nNOS. All neurons containing nNOS in rat striatum were found to contain NADPHd. Combining our various quantitative observations, we found that of those striatal neurons containing any combination of SS, NPY, nNOS and NADPHd in rats, about 73% contained all four, 16% contained SS, nNOS and NADPHd, 5% contained SS only, and 6% contained only nNOS and NADPHd. These results indicate that while there is a large population of striatal neurons in which SS, NPY, nNOS and NADPHd are colocalized in rats, there may be smaller populations of striatal neurons devoid of NPY in which SS or nNOS/NADPHd are found individually or together.