Characterization of alpha and beta tubulin genes in the dimorphic fungus Histoplasma capsulatum.
Characterization of alpha and beta tubulin genes in the dimorphic fungus Histoplasma capsulatum.
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二形性真菌荚膜组织胞浆菌中α和β微管蛋白基因的表征。
DOI：
10.1099/00221287-135-7-1817
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Medoff,J
中科院分区：
文献类型：
--
作者：
Harris,GS;Keath,EJ;Medoff,J
来自我们实验室的证据表明，微管参与的二型，致病真菌荚膜组织胞浆菌的分化，因此，我们克隆了微管蛋白基因的生物体的强毒株。我们报告说，H。荚膜基因组包含单个α（TUB1）和单个β（TUB2）微管蛋白基因，而不是在甚至最简单的真核生物中常见的更典型的多基因家族。来自这些基因的序列数据揭示了相对于来自其他物种的微管蛋白的高度核苷酸和蛋白质序列保守性。TUB1和TUB2的编码区分别含有5个和8个插入序列。场反转凝胶电泳。荚膜染色体大小的DNA片段表明TUB1和TUB2基因是不连锁的。两种基因共同的潜在调节元件已在5 '启动子区被鉴定出来。这些元件可能指导TUB1和TUB2在分化过程中的协调表达。从H. capsulatum首次描述了这种广泛分布的致病真菌的基因结构。微管蛋白基因的分离将有助于进一步研究微管蛋白基因在二态性相变过程中的表达，并阐明微管在分化过程中的作用。
Evidence from our laboratory indicates that microtubules are involved in the differentiation of the dimorphic, pathogenic fungusHistoplasma capsulatum; therefore, we cloned the tubulin genes from a virulent strain of the organism. We report that theH. capsulatumgenome contains a single alpha (TUB1) and a single beta (TUB2) tubulin gene rather than the more typical multigene family which is common in even the simplest eukaryotes. Sequence data from these genes reveal a high degree of nucleotide and protein sequence conservation relative to tubulins from other species. The coding regions ofTUB1andTUB2contain five and eight intervening sequences, respectively. Field inversion gel electrophoresis ofH. capsulatumchromosome-sized DNA fragments indicates that theTUB1andTUB2genes are unlinked. Potential regulatory elements common to both genes have been identified in the 5′ promoter regions. These elements may direct the coordinate expression ofTUB1andTUB2during differentiation. The cloning and characterization of alpha and beta tubulin genes fromH. capsulatumprovides the first description of gene structure in this widely distributed pathogenic fungus. Isolation of the tubulin genes will facilitate future studies of tubulin gene expression during the dimorphic phase transitions and clarify the role of microtubules in the differentiation process.