Comparative genomic sequence analysis of the Marek's disease vaccine strain SB-1
Comparative genomic sequence analysis of the Marek's disease vaccine strain SB-1
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DOI：
10.1007/s11262-011-0573-0
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发表时间：
2011-06-01
期刊：
影响因子：
1.6
通讯作者：
Schat, Karel A.
中科院分区：
文献类型：
--
作者：
Spatz, Stephen J.;Schat, Karel A.
马立克氏病病毒（MDV）是一种致癌的α疱疹病毒，可引起鸡的快速发病的T细胞淋巴瘤和脱髓鞘疾病。自20世纪70年代以来，通过大规模接种火鸡疱疹病毒[火鸡疱疹病毒1型（MeHV-1）]控制了该病。随着时间的推移，这种疫苗的效力下降，并且在20世纪80年代引入了由MeHV-1和称为SB-1的非致癌性鸡疱疹病毒3型（GaHV-3）菌株组成的二价疫苗。使用454焦磷酸测序法测定该GaHV-3菌株的完整DNA序列（165，994 bp）。使用BLAST（E值< 0.9）检查总共524个开放阅读框（ORF）与GenBank中存在的蛋白质序列的同源性。在128个ORF命中中，75个ORF显示与充分表征的α疱疹病毒蛋白同源。在系统发生学上，该毒株与GaHV-3毒株HPRS 24一起在其自身的分支中划分，并且显示出比鸡疱疹病毒2型（GaHV-2，马立克氏病病毒）与MeHV-1更相关。将GaHV-3的ORF与MeHV-1和GaHV-2中的同源ORF进行比较时，发现SB-1基因组中的同源ORF的氨基酸相似性百分比高于HPRS 24基因组中的同源ORF。总体而言，SB-1基因组内的75个ORF中有两倍多的ORF显示出与马立克氏病基因组中的同源ORF相比HPRS 24基因组中的同源ORF更大的序列同一性和相似性。本文描述了两种GaHV-3基因组之间的序列差异。总体上，19个ORF在预测的氨基酸数目上不同;其中，八个（U（L）3.5，U（L）5，U（L）9，U（L）28，U（L）30，U（L）36，U（L）37，和U（L）50）编码充分表征的α疱疹病毒蛋白A序列在SB-1基因组的独特短区域内表现出与长末端重复序列（LTR）显著的序列同源性，鸟类逆转录病毒的序列。该序列仅在SB-1基因组中发现，而在HPRS 24基因组中未发现。
Marek's disease virus (MDV), an oncogenic alphaherpesvirus, induces a rapid onset T-cell lymphoma and demyelinating disease in chickens. Since the 1970s the disease has been controlled through mass vaccination with herpesvirus of turkeys [meleagrid herpesvirus type 1 (MeHV-1)]. Over time this vaccine's efficacy decreased, and in the 1980s a bivalent vaccine consisting of MeHV-1 and a non-oncogenic gallid herpesvirus type 3 (GaHV-3) strain known as SB-1 was introduced. The complete DNA sequence (165,994 bp) of this GaHV-3 strain was determined using 454 pyrosequencing. A total of 524 open reading frames (ORFs) were examined for homology to protein sequences present in GenBank using BLAST (E-values < 0.9). Of the 128 ORF hits, 75 ORFs showed homology to well-characterized alphaherpesviral proteins. Phylogenetically, this strain partitions in its own branch along with the GaHV-3 strain HPRS24 and shows more relatedness to MeHV-1 than gallid herpesvirus type 2 (GaHV-2, Marek's disease virus). When comparing the GaHV-3 ORFs to their homologues in MeHV-1 and GaHV-2, a greater percentage of amino acid similarity was found with homologous ORFs in the genome of SB-1 than with those in the HPRS24 genome. Overall, twice as many of the 75 ORFs within the SB-1 genome showed greater sequence identities and similarities to homologous ORFs in the Marek's disease genome than those within the HPRS24 genome. This paper describes the sequence difference between the two GaHV-3 genomes. Overall 19 ORFs differ in the number of predicted amino acids; of these, eight (U(L)3.5, U(L)5, U(L)9, U(L)28, U(L)30, U(L)36, U(L)37, and U(L)50) encode well-characterized alphaherpesviral proteins A sequence within the unique short region of the SB-1 genome exhibited significant sequence homology to long terminal repeat (LTR) sequences of avian retroviruses. This sequence was only found in the SB-1 genome and not the HPRS24 genome.