Sox genes regulate type 2 collagen expression in avian neural crest cells
Sox genes regulate type 2 collagen expression in avian neural crest cells
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DOI：
10.1111/j.1440-169x.2006.00886.x
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发表时间：
2006-10-01
影响因子：
2.5
通讯作者：
Wakamatsu, Yoshio
中科院分区：
文献类型：
--
作者：
Suzuki, Takashi;Sakai, Daisuke;Wakamatsu, Yoshio
神经脊细胞产生各种各样的细胞类型，包括颅骨中的软骨细胞和周围神经系统中的神经元和神经胶质细胞。为了探讨软骨分化和神经脊分化的关系，我们检测了编码经常被用作软骨标志物的2型胶原的COL2a1的表达，并与参与神经脊发育和软骨形成的SOX转录因子基因的表达进行了比较。我们发现，随着Sox9、Sox10和LSox5的结合，Col2a1在许多神经嵴来源的细胞类型中都有表达。过表达研究表明，Sox9和Sox10激活了Col2a1的表达，并对这些Sox基因进行了交叉调节。荧光素酶分析表明，Sox9和Sox10都能直接激活Col2a1增强子/启动子，这种激活被cAMP依赖的激酶(PKA)信号进一步增强。我们的研究表明，软骨和神经脊分化的调节机制是相似的。
Neural crest cells give rise to a wide variety of cell types, including cartilage cells in the cranium and neurons and glial cells in the peripheral nervous system. To examine the relationship of cartilage differentiation and neural crest differentiation, we examined the expression of Col2a1, which encodes type 2 collagen often used as a cartilage marker, and compared it with the expression of Sox transcription factor genes, which are involved in neural crest development and chondrogenesis. We found that Col2a1 is expressed in many neural crest-derived cell types along with combinations of Sox9, Sox10 and LSox5. Overexpression studies reveal the activation of Col2a1 expression by Sox9 and Sox10, and cross-regulation of these Sox genes. Luciferase assay indicates a direct activation of the Col2a1 enhancer/promoter both by Sox9 and Sox10, and this activation is further enhanced by cAMP-dependent kinase (PKA) signaling. Our study suggests that the regulatory mechanisms are similar in cartilage and neural crest differentiation.