STRUCTURE, CHROMOSOMAL LOCALIZATION, AND EXPRESSION OF 12 GENES OF THE MAGE FAMILY
STRUCTURE, CHROMOSOMAL LOCALIZATION, AND EXPRESSION OF 12 GENES OF THE MAGE FAMILY
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DOI：
10.1007/bf01246677
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发表时间：
1994-09-01
期刊：
影响因子：
3.2
通讯作者：
BOON, T
中科院分区：
文献类型：
--
作者：
DEPLAEN, E;ARDEN, K;BOON, T
我们以前报道过人类基因法师-1指导自体溶细胞性T淋巴细胞在黑色素瘤上识别的肿瘤抗原的表达。用法师-1序列检测粘粒文库，我们鉴定了11个密切相关的基因。对仓鼠-人体细胞杂种的分析表明，这12个法师基因均位于X染色体q端。与法师-1一样，另外11个法师基因的全部编码序列位于最后一个外显子，与法师-1的编码序列具有64%-85%的同源性。法师基因的编码序列预测了所有法师蛋白相同的主要结构特征。相反，12个法师基因的启动子和第一外显子显示出相当大的变异性，这表明该基因家族的存在使得相同的功能能够在不同的转录控制下表达。通过逆转录和聚合酶链反应扩增评价每个法师基因的表达。发现包括法师-1在内的法师家族的6个基因在多种组织学类型的肿瘤中高水平表达。除了睾丸和胎盘外，在大量健康组织中均不表达。
We reported previously that human gene MAGE-1 directs the expression of a tumor antigen recognized on a melanoma by autologous cytolytic T lymphocytes. Probing cosmid libraries with a MAGE-1 sequence, we identified 11 closely related genes. The analysis of hamster-human somatic cell hybrids indicated that the 12 MAGE genes are located in the q terminal region of chromosome X. Like MAGE-1, the 11 additional MAGE genes have their entire coding sequence located in the last exon, which shows 64%-85% identity with that of MAGE-1. The coding sequences of the MAGE genes predict the same main structural features for all MAGE proteins. In contrast, the promoters and first exons of the 12 MAGE genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The expression of each MAGE gene was evaluated by reverse transcription and polymerase chain reaction amplification. Six genes of the MAGE family including MAGE-1 were found to be expressed at a high level in a number of tumors of various histological types. None was expressed in a large panel of healthy tissues, with the exception of testis and placenta.