Characterization of a novel EGFP reporter mouse to monitor Cre recombination as demonstrated by a Tie2 Cre mouse line
Characterization of a novel EGFP reporter mouse to monitor Cre recombination as demonstrated by a Tie2 Cre mouse line
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DOI：
10.1002/gene.1030
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发表时间：
2001-05-01
期刊：
影响因子：
1.5
通讯作者：
Arnold, B
中科院分区：
文献类型：
--
作者：
Constien, R;Forde, A;Arnold, B
Cre/loxP系统的使用极大地增强了基因靶向领域的能力。在这里，我们描述了一个新的基因敲入的EGFP报告小鼠系，以监测矿石诱导的重组，在绝大多数细胞类型的成功建立。该报告小鼠系的价值通过使用促进内皮细胞和造血细胞中的基因靶向的新型Tie 2Cre转基因小鼠系来证明。在所有内皮细胞和大多数造血细胞中发现了高重组效率，但在其他组织中不存在。此外，在第二代中，Tie 2Cre小鼠可用于获得一个等位基因的100%重组，同时允许第二代中的组织特异性，因此提供优异的效率。创世纪30：36- 44，2001。(C)2001 Wiley-Liss，Inc.
The use of the Cre/loxP system has greatly empowered the field of gene targeting. Here we describe the successful establishment of a novel knock-in EGFP reporter mouse line to monitor ore-induced recombination in the vast majority of cell types. The value of this reporter mouse line is demonstrated by the use of a novel Tie2Cre transgenic mouse line that facilitates gene targeting in endothelial and hematopoietic cells, High efficiency of recombination was found in all endothelial cells and in the majority of hematopoietic cells but was absent in other tissues. Furthermore, in the second generation, the Tie2Cre mouse can be used to get 100% recombination of one allele, whilst allowing tissue specific in the second, therefore offering excellent efficiency. genesis 30:36-44,2001. (C) 2001 Wiley-Liss, Inc.