Functional mapping of bluetongue virus proteins and their interactions with host proteins during virus replication
Functional mapping of bluetongue virus proteins and their interactions with host proteins during virus replication
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DOI：
10.1007/s12013-008-9009-4
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发表时间：
2008-03-01
影响因子：
2.6
通讯作者：
Roy, Polly
中科院分区：
文献类型：
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作者：
Roy, Polly
蓝舌病病毒（BTV）是一种双链RNA（dsRNA）病毒，通过吸血蚊虫传播给野生和家养反刍动物，造成高发病率和高死亡率。部分原因在于，BTV在过去三十年中一直处于分子研究的前沿，现在代表了分子和结构水平上最好理解的病毒之一。与呼肠孤病毒科的其他成员一样，BTV是一种复杂的无包膜病毒，具有7种结构蛋白和由10个不同大小的dsRNA片段组成的RNA基因组。在病毒感染的细胞中，合成三种其他病毒编码的非结构蛋白。近年来，BTV蛋白的结构与功能关系及其在病毒组装过程中的相互作用研究取得了重要进展。通过结合结构和分子数据，有可能在病毒入侵、复制和逃离易感宿主细胞的基本机制方面取得进展。从多年的研究中获得的数据已经确定了BTV进入，复制，组装和出口的关键参与者。具体而言，已经可以通过三维结构重建确定病毒粒子的复杂性质;蛋白质和内部衣壳的原子结构; RNA复制所需的病毒编码酶的定义;衣壳壳的有序组装和所需的蛋白质螯合;以及三种NS蛋白在病毒复制，组装和释放中的作用。总体而言，这一审查表明，结构，生化和分子数据的整合是必要的，以充分了解这种复杂的RNA病毒的组装和复制。
Bluetongue virus (BTV) is a double-stranded RNA (dsRNA) virus which is transmitted by blood-feeding gnats to wild and domestic ruminants, causing high morbidity and often high mortality. Partly due to this BTV has been in the forefront of molecular studies for last three decades and now represents one of the best understood viruses at the molecular and structural levels. BTV, like the other members of the Reoviridae family is a complex non-enveloped virus with seven structural proteins and a RNA genome consisting of 10 dsRNA segments of different sizes. In virus infected cells, three other virus encoded nonstructural proteins are synthesized. Significant recent advances have been made in understanding the structure-function relationships of BTV proteins and their interactions during virus assembly. By combining structural and molecular data it has been possible to make progress on the fundamental mechanisms used by the virus to invade, replicate in, and escape from, susceptible host cells. Data obtained from studies over a number of years have defined the key players in BTV entry, replication, assembly and egress. Specifically, it has been possible to determine the complex nature of the virion through three dimensional structure reconstructions; atomic structure of proteins and the internal capsid; the definition of the virus encoded enzymes required for RNA replication; the ordered assembly of the capsid shell and the protein sequestration required for it; and the role of three NS proteins in virus replication, assembly and release. Overall, this review demonstrates that the integration of structural, biochemical and molecular data is necessary to fully understand the assembly and replication of this complex RNA virus.