Genetic diversity among Borrelia strains determined by single-strand conformation polymorphism analysis of the ospC gene and its association with invasiveness
Genetic diversity among Borrelia strains determined by single-strand conformation polymorphism analysis of the ospC gene and its association with invasiveness
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DOI：
10.1128/jcm.41.11.5059-5065.2003
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发表时间：
2003-11-01
影响因子：
9.4
通讯作者：
Baranton, G
中科院分区：
文献类型：
--
作者：
Lagal, V;Postic, D;Baranton, G
莱姆疏螺旋体病 (LB) 是一种蜱传螺旋体感染，由三种疏螺旋体引起：阿氏疏螺旋体、加氏疏螺旋体和严格伯氏疏螺旋体。 LB 分为两个阶段：称为游走性红斑的皮肤病变，以及后来的不同播散形式（关节、神经、心脏......）。先前基于 ospC 序列分析的研究允许定义 58 个组（组间差异为 8%）。其中只有 10 个组包含从被认为具有侵入性的传播形式中分离出的所有菌株。本研究的目的是通过测试从传播形式中分离出的人类临床菌株来确定侵入菌株是否属于限制性 ospC 群体。为了筛选 ospC 遗传多样性，我们使用单链构象多态性 (SSCP) 分析。首先测试了来自 44 个不同菌株的先前已知的 ospC 序列，揭示了每个 ospC 组都具有特征性的 SSCP 模式。因此，我们研究了 80 个 ospC 序列未知的播散型分离株。其中，28 个（35%）属于先前已知的入侵群体。此外，还确定了新的入侵类群：6 个 B. afield、7 个 B. garinii 和 1 个 B. burgdorferi sensu stricto。这项研究证实，入侵菌株并不分布在所有 69 个 ospC 群体中，而是只属于 24 个群体。这表明OspC可能参与伯氏疏螺旋体的侵袭。
Lyme borreliosis (LB) is a tick-borne spirochetal infection caused by three Borrelia species: Borrelia afzelii, B. garinii, and B. burgdorferi sensu stricto. LB evolves in two stages: a skin lesion called erythema migrans and later, different disseminated forms (articular, neurological, cardiac...). Previous research based on analysis of ospC sequences allowed the definition of 58 groups (divergence of 8% between groups). Only 10 of these groups include all of the strains isolated from disseminated forms that are considered invasive. The aim of this study was to determine whether or not invasive strains belong to restricted ospC groups by testing human clinical strains isolated from disseminated forms. To screen for ospC genetic diversity, we used single-strand conformation polymorphism (SSCP) analysis. Previously known ospC sequences from 44 different strains were first tested, revealing that each ospC group had a characteristic SSCP pattern. Therefore, we studied 80 disseminated-form isolates whose ospC sequences were unknown. Of these, 28 (35%) belonged to previously known invasive groups. Moreover, new invasive groups were identified: six of B. afield, seven of B. garinii, and one of B. burgdorferi sensu stricto. This study confirmed that invasive strains are not distributed among all 69 ospC groups but belong to only 24 groups. This suggests that OspC may be involved in the invasiveness of B. burgdorferi.