Optically controlled contraction of photosensitive skeletal muscle cells
Optically controlled contraction of photosensitive skeletal muscle cells
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DOI：
10.1002/bit.23285
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发表时间：
2012-01-01
影响因子：
3.8
通讯作者：
Yawo, Hiromu
中科院分区：
文献类型：
--
作者：
Asano, Toshifumi;Ishizua, Toru;Yawo, Hiromu
由于骨骼肌细胞是一种有效的力传感器，它已被纳入生物微器件使用电场刺激产生收缩模式。为了提高空间和时间分辨率，我们从小鼠C2 C12成肌细胞中制备了光敏骨骼肌细胞，其表达通道视紫红质-2（ChR 2），ChR 2是来自绿色藻类莱茵衣藻的古菌型视紫红质之一。制备克隆的表达ChR 2的C2 C12成肌细胞，并与未转染的C2 C12融合以形成多核肌管。电场刺激促进了肌管的成熟。蓝色LED光脉冲使表达ChR 2的肌管的膜电位去极化，并最终诱发动作电位。它还同时诱导了抽搐样收缩。因此，利用给定的LED脉冲图案形成收缩图案。该技术在生物工程领域有许多应用，例如肌肉驱动的致动器/微器件的无线驱动。Biotechnol. Bioeng. 2012;109：199204. (c)2011 Wiley Periodicals，Inc.
As the skeletal muscle cell is an efficient force transducer, it has been incorporated in bio-microdevices using electrical field stimulation for generating contractile patterns. To improve both the spatial and temporal resolutions, we made photosensitive skeletal muscle cells from murine C2C12 myoblasts, which express channelrhodopsin-2 (ChR2), one of archaea-type rhodopsins derived from green algae Chlamydomonas reinhardtii. The cloned ChR2-expressing C2C12 myoblasts were made and fused with untransfected C2C12 to form multinucleated myotubes. The maturation of myotubes was facilitated by electrical field stimulation. Blue LED light pulse depolarized the membrane potential of a ChR2-expressing myotube and eventually evoked an action potential. It also induced a twitch-like contraction in a concurrent manner. A contraction pattern was thus made with a given pattern of LED pulses. This technique would have many applications in the bioengineering field, such as wireless drive of muscle-powered actuators/microdevices. Biotechnol. Bioeng. 2012;109: 199204. (c) 2011 Wiley Periodicals, Inc.