Aberrant promoter methylation of PTEN gene among Indian patients with oral squamous cell carcinoma
Aberrant promoter methylation of PTEN gene among Indian patients with oral squamous cell carcinoma
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DOI：
10.5301/jbm.5000030
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发表时间：
2013-07-01
影响因子：
2
通讯作者：
Rizvi, Moshahid A.
中科院分区：
文献类型：
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作者：
Alyasiri, Nisreen S.;Ali, Asgar;Rizvi, Moshahid A.
背景和目的：口腔癌是男性第二常见的癌症，占印度癌症总死亡率的50%-70%。PTEN是一种肿瘤抑制基因，在控制细胞生长和存活中起关键作用。在许多肿瘤中已经报道了PTEN基因的启动子高甲基化。然而，很少有人知道启动子甲基化和口腔鳞状细胞癌（OSCC）之间的关联。因此，我们的目的是检测在印度population.Methods口腔鳞癌患者的PTEN高甲基化的作用：基因组DNA从100个新鲜的口腔肿瘤标本中分离，并进行亚硫酸氢盐转换。甲基化特异性PCR对转化的DNA进行甲基化状态调查。结果：在所有检查的病例中，我们发现35%的PTEN启动子甲基化阳性，65%为阴性。当评估与肿瘤分化，它被发现，82%的低分化，24%的中度分化和32%的分化良好的口腔鳞状细胞癌样本甲基化。甲基化还与患者的年龄、性别和肿瘤分级相关。有趣的是，我们发现，患者的年龄和分化程度与PTEN启动子甲基化显着相关（p=0.05和0.0019，分别），而性别不是（p=0.9）。结论：本研究强调了印度口腔鳞癌患者中PTEN高甲基化的重要性。
Background and aims: Oral cancer is the second most common cancer in men and accounts for 50%-70% of the total cancer mortality in India. PTEN is a tumor suppressor gene that plays a critical role in controlling cell growth and survival. Promoter hypermethylation of the PTEN gene has been reported in many tumors. However, little is known about the association between promoter methylation and oral squamous cell carcinoma (OSCC). Therefore, we aimed to detect the role of PTEN hypermethylation in OSCC patients in the Indian population.Methods: Genomic DNA was isolated from 100 fresh oral tumor specimens and was subjected to bisulfite conversion. Methylation-specific PCR was employed on the converted DNA to investigate the methylation status.Results: Of the total cases examined for PTEN promoter methylation we found that 35% were positive and 65% were negative. When evaluated in connection with tumor differentiation it was found that 82% of poorly differentiated, 24% of moderately differentiated and 32% of well differentiated OSCC samples were methylated. Methylation was further correlated with patient age, sex and tumor grade. Interestingly, we found that patient age and grade of differentiation were significantly associated with PTEN promoter methylation (p=0.05 and 0.0019, respectively) while sex was not (p=0.9).Conclusions: The present study underlines the importance of PTEN hypermethylation among Indian OSCC patients.