Comparison of platelet activation in platelet concentrates measured by flow cytometry or ADVIA 2120
Comparison of platelet activation in platelet concentrates measured by flow cytometry or ADVIA 2120
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DOI：
10.1111/j.1423-0410.2011.01469.x
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发表时间：
2011-08-01
期刊：
影响因子：
2.7
通讯作者：
Thomas, S.
中科院分区：
文献类型：
--
作者：
Beard, M. J.;Jeewa, Z.;Thomas, S.
背景和目的ADVIA 2120血液分析仪能够测量可用作血小板活化标志物的参数，即平均血小板成分（MPC）、血小板成分分布宽度（PCDW）和平均血小板质量（MPM）。本研究探讨了这些措施的血小板粒度与CD62 P测量血小板活化的流式细胞术在plateletcretreat.Materials和方法合并血小板血浆/柠檬酸盐磷酸盐葡萄糖（CPD）抗凝剂或单采血小板血浆/酸柠檬酸盐葡萄糖公式A（ACD-A）抗凝剂的相关程度进行了评价。在13天储存期间检测合并的血小板，并在静脉穿刺后24小时内检测单采血小板。这些进行了评估，血小板活化使用CD62 P和ADVIA，有或没有额外的EDTA抗凝剂。结果在合并的血小板，PCDW与CD62 P，有和没有额外的EDTA抗凝剂。在添加EDTA的情况下，MPC和CD 62 P之间具有良好的相关性，但在不添加EDTA的情况下，MPC和CD 62 P之间的相关性较弱。CD 62 P与MPM无相关性。在单采血小板的PCDW和CD 62 P之间的相关性较差，而MPC与CD 62 P强相关，如果EDTA抗凝剂added.Conclusion的有用性ADVIA血小板粒度的措施来预测血小板活化的程度取决于抗凝剂存在于血小板浓缩物，以及是否额外的EDTA被添加到样品。虽然ADVIA MPC和PCDW测量不能取代CD62P或其他评估血小板活化的金标准方法，但这些ADVIA 2120参数可提供血小板浓缩物质量的快速检查。
Background and Objectives The ADVIA 2120 Haematology Analyser is capable of measuring parameters that can be used as markers of platelet activation, mean platelet component (MPC), platelet component distribution width (PCDW) and mean platelet mass (MPM). This study investigated the degree of correlation of these measures of platelet granularity with CD62P measurement of platelet activation by flow cytometry in platelet concentrates.Materials and Methods Pooled platelets in plasma/citrate phosphate dextrose (CPD) anticoagulant or apheresis platelets in plasma/acid citrate dextrose formula A (ACD-A) anticoagulant were evaluated. Pooled platelets were tested during 13 day storage, and apheresis platelets within 24 h of venepuncture. These were assessed for platelet activation using CD62P and the ADVIA, with or without extra EDTA anticoagulant.Results In pooled platelets, PCDW correlated strongly with CD62P, both with and without the addition of extra EDTA anticoagulant. There was a good correlation between MPC and CD62P with additional EDTA, but a weaker correlation without extra EDTA. There was no correlation between CD62P and MPM. In apheresis platelets the correlation between PCDW and CD62P was poor, whereas MPC correlated strongly with CD62P if EDTA anticoagulant was added.Conclusion The usefulness of ADVIA platelet granularity measures to predict the degree of platelet activation depends upon the anticoagulant present in the platelet concentrate, and whether extra EDTA is added to the sample. Although ADVIA MPC and PCDW measurement could not replace CD62P or other gold standard methods of assessing platelet activation, these ADVIA 2120 parameters may provide a quick check of platelet concentrate quality.