Extraction and Characterization of Lipids from Macroalgae
Extraction and Characterization of Lipids from Macroalgae
复制标题
大型藻类脂质的提取和表征
DOI：
10.1007/978-1-4939-9484-7_7
复制
发表时间：
2019
影响因子：
--
通讯作者：
Nobles DR Jr, Manning SR
中科院分区：
文献类型：
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作者：
Nobles DR Jr, Manning SR
虽然大多数藻类生物燃料研究都集中在微藻上，但大型藻类也是生产生物柴油和其他液体燃料的潜在脂质来源。可靠，准确的方法来评估生物量的脂质组成是必不可少的大型藻类在这一领域的发展。最常用于评价脂质组成的常规方法，例如Bligh和Dyer和Folch的方法，不能完全提取光合细胞/组织中的脂质，因此不能准确计算脂质产生。在这里，我们提出了一个2-EE脂质提取协议，已被证明是上级的微藻，适用于与大型藻类的常规脂质提取方法的方法。
Although most algal biofuel research has focused on microalgae, macroalgae are also potential sources of lipid for the production of biodiesel and other liquid fuels. Reliable, accurate methods for assessing the lipid composition of biomass are essential for the development of macroalgae in this area. The conventional methods most commonly used to evaluate lipid composition, such as those of Bligh and Dyer and Folch, do not provide complete extraction of lipids in photosynthetic cells/tissues and therefore do not provide an accurate accounting of lipid production. Here we present a 2-EE lipid extraction protocol, a method which has been demonstrated to be superior to conventional lipid extraction methods for microalgae, adapted for use with macroalgae.