Gene expression analysis of hypersensitivity to mosquito bite, chronic active EBV infection and NK/T-lymphoma/leukemia
Gene expression analysis of hypersensitivity to mosquito bite, chronic active EBV infection and NK/T-lymphoma/leukemia
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DOI：
10.1080/10428194.2017.1304762
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发表时间：
2017-01-01
影响因子：
2.6
通讯作者：
Tsukahara, Hirokazu
中科院分区：
文献类型：
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作者：
Washio, Kana;Oka, Takashi;Tsukahara, Hirokazu
人类疱疹病毒爱泼斯坦-巴尔病毒（EBV）是一种已知的致癌病毒，在危及生命的T/NK细胞淋巴增殖性疾病（T/NK细胞LPD）中起重要作用，如对蚊虫叮咬的过敏反应（HMB）、慢性活动性EBV感染（CAEBV）和NK/T细胞淋巴瘤/白血病。在HMB和CAEBV的临床病程中，患者经常发生恶性淋巴瘤，疾病被动进展。在本研究中，利用寡核苷酸芯片分析了从患者身上建立的CD16（(-)）、CD56((+))-、EBV(+) HMB、CAEBV、NK淋巴瘤和NK白血病细胞系的基因表达，并与来自健康供体的CD56（亮）CD16（暗/-）NK细胞的基因表达进行了比较。主成分分析表明，CAEBV和nk -淋巴瘤细胞的位置相对较近，表明它们具有相似的表达谱。微阵列数据的无监督分层聚类分析和基因本体分析揭示了特定的基因簇，并确定了几个与疾病相关的候选基因，这些基因可用于区分NK-LPD和nk细胞淋巴瘤/白血病的各个类别。
The human herpes virus, Epstein-Barr virus (EBV), is a known oncogenic virus and plays important roles in life-threatening T/NK-cell lymphoproliferative disorders (T/NK-cell LPD) such as hypersensitivity to mosquito bite (HMB), chronic active EBV infection (CAEBV), and NK/T-cell lymphoma/leukemia. During the clinical courses of HMB and CAEBV, patients frequently develop malignant lymphomas and the diseases passively progress sequentially. In the present study, gene expression of CD16((-))CD56((+))-, EBV(+) HMB, CAEBV, NK-lymphoma, and NK-leukemia cell lines, which were established from patients, was analyzed using oligonucleotide microarrays and compared to that of CD56(bright)CD16(dim/-) NK cells from healthy donors. Principal components analysis showed that CAEBV and NK-lymphoma cells were relatively closely located, indicating that they had similar expression profiles. Unsupervised hierarchal clustering analyses of microarray data and gene ontology analysis revealed specific gene clusters and identified several candidate genes responsible for disease that can be used to discriminate each category of NK-LPD and NK-cell lymphoma/leukemia.