The SERRATE protein is involved in alternative splicing in Arabidopsis thaliana.
The SERRATE protein is involved in alternative splicing in Arabidopsis thaliana.
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DOI：
10.1093/nar/gkt894
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发表时间：
2014-01
影响因子：
14.9
通讯作者：
Jarmolowski A
中科院分区：
文献类型：
--
作者：
Raczynska KD;Stepien A;Kierzkowski D;Kalak M;Bajczyk M;McNicol J;Simpson CG;Szweykowska-Kulinska Z;Brown JW;Jarmolowski A
植物中选择性剪接（AS）是如何调控的尚未阐明。在此之前，我们已经证明核帽结合蛋白复合物（AtCBC）参与拟南芥的AS。在这里，我们发现AtCBC的两个亚基（AtCBP20和AtCBP80）与SERRATE （AtSE）相互作用，AtSE是一种参与microRNA生物发生途径的蛋白质。此外，使用高分辨率逆转录聚合酶链反应AS系统，我们发现AtSE影响AS的方式与帽结合复合体（CBC）相似，优先影响第一个内含子的5 '剪接位点的选择。AtSE蛋白与AtCBC协同作用：在缺乏正确SERRATE活性的突变体中观察到的许多变化与在cbp突变体中观察到的变化相同。有趣的是，在植物microRNA生物发生途径的其他突变体hyl1-2和dcl1-7中也观察到一些基因的AS发生了显著变化，但大多数与se-1突变体的变化不一致。因此，SERRATE在AS调控中的作用不同于HYL1和DCL1，而与CBC参与的AS调控相似。
How alternative splicing (AS) is regulated in plants has not yet been elucidated. Previously, we have shown that the nuclear cap-binding protein complex (AtCBC) is involved in AS in Arabidopsis thaliana. Here we show that both subunits of AtCBC (AtCBP20 and AtCBP80) interact with SERRATE (AtSE), a protein involved in the microRNA biogenesis pathway. Moreover, using a high-resolution reverse transcriptase-polymerase chain reaction AS system we have found that AtSE influences AS in a similar way to the cap-binding complex (CBC), preferentially affecting selection of 5′ splice site of first introns. The AtSE protein acts in cooperation with AtCBC: many changes observed in the mutant lacking the correct SERRATE activity were common to those observed in the cbp mutants. Interestingly, significant changes in AS of some genes were also observed in other mutants of plant microRNA biogenesis pathway, hyl1-2 and dcl1-7, but a majority of them did not correspond to the changes observed in the se-1 mutant. Thus, the role of SERRATE in AS regulation is distinct from that of HYL1 and DCL1, and is similar to the regulation of AS in which CBC is involved.