Reappraisal of the taxonomy of Streptococcus suis serotypes 20, 22 and 26: Streptococcus parasuis sp nov.
Reappraisal of the taxonomy of Streptococcus suis serotypes 20, 22 and 26: Streptococcus parasuis sp nov.
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DOI：
10.1099/ijs.0.067116-0
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发表时间：
2015-02-01
影响因子：
2.8
通讯作者：
Osawa, Ro
中科院分区：
文献类型：
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作者：
Nomoto, R.;Maruyama, F.;Osawa, Ro
为明确猪链球菌20、22和26血清型的分类地位，对分离株SUT-7、SUT-286(T)、SUT-319、SUT-328和SUT-380与健康猪唾液中20、22和26血清型特异性抗血清以及20、22和26血清型参比株进行了生化和分子遗传学研究。比较recN基因测序结果显示，各分离株具有较高的遗传亲缘性，但与猪链球菌NCTC 10234(T)的序列相似度为74.8 ~ 75.7%，差异显著。利用全基因组序列的平均核苷酸同一性值计算了分离株与猪链球菌属其他菌株的基因组亲缘性，结果表明血清型20、22和26应从猪链球菌中分离出来，并作为一个新的基因组种处理。对比序列分析显示，血清型20、22和26参考菌株与分离株的16S rRNA基因序列相似性为99.0 ~ 100%。分离株stu286 (T)与猪链球菌NCTC 10234(T)的16S rRNA基因序列相似性较高（98.8%）。SUT-286(T)可与猪链球菌及其他近缘种链球菌进行生化鉴定。由于其系统发育和表型与猪链球菌相似，我们建议将该新种命名为副猪链球菌sp. nov.，并以SUT-286(T) (=JCM 30273（T)=DSM 29126(T)）为型菌株。
In order to clarify the taxonomic position of serotypes 20, 22 and 26 of Streptococcus suis, biochemical and molecular genetic studies were performed on isolates (SUT-7, SUT-286(T), SUT-319, SUT-328 and SUT-380) reacted with specific antisera of serotypes 20, 22 or 26 from the saliva of healthy pigs as well as reference strains of serotypes 20, 22 and 26. Comparative recN gene sequencing showed high genetic relatedness among our isolates, but marked differences from the type strain S. suis NCTC 10234(T), i.e. 74.8-75.7% sequence similarity. The genomic relatedness between the isolates and other strains of species of the genus Streptococcus, including S. suis, was calculated using the average nucleotide identity values of whole genome sequences, which indicated that serotypes 20, 22 and 26 should be removed taxonomically from S. suis and treated as a novel genomic species. Comparative sequence analysis revealed 99.0-100 % sequence similarities for the 16S rRNA genes between the reference strains of serotypes 20, 22 and 26, and our isolates. Isolate STU-286(T) had relatively high 16S rRNA gene sequence similarity with S. suis NCTC 10234(T) (98.8%). SUT-286(T) could be distinguished from S. suis and other closely related species of the genus Streptococcus using biochemical tests. Due to its phylogenetic and phenotypic similarities to S. suis we propose naming the novel species Streptococcus parasuis sp. nov., with SUT-286(T) (=JCM 30273(T)=DSM 29126(T)) as the type strain.