Dietary fish oil and cytokine and eicosanoid production during human immunodeficiency virus infection
Dietary fish oil and cytokine and eicosanoid production during human immunodeficiency virus infection
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DOI：
10.1177/014860719602000143
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发表时间：
1996-01-01
影响因子：
3.4
通讯作者：
Forse, Tohru Utsunomyia Annd R. Armour
中科院分区：
文献类型：
--
作者：
Bell, Stacy J.;Chavali, Sambasiva;Forse, Tohru Utsunomyia Annd R. Armour
背景膳食鱼油（FO）已被证明可以调节免疫系统。本研究的目的是探讨FO补充对人类免疫缺陷病毒（HIV）感染患者产生的二烯类二十烷酸和细胞因子的影响。方法：这是一项随机、前瞻性、双盲研究，包括HIV感染的同性恋男性。患者被要求每天自愿食用5个含有FO（n = 10）或红花油（SO）（n = 9）的食物棒，持续6周。在基线和第6周，获得血浆以测量ω-3脂肪酸的掺入。在基线、第3周和第6周，测量脂多糖（LPS）刺激的外周血单核细胞（PBMC）或自发释放细胞中二烯类二十烷酸和细胞因子的变化。结果如下：在FO组中，而不是SO组中，ω-3脂肪酸二十二碳六烯酸（DHA）掺入到血浆脂肪酸的磷脂中增加。在FO组中，PBMC释放的6-酮前列腺素（PG）F-1 α显著减少（p = 0.01）。在基线和第3周之间以及第3周和第6周之间，FO组PBMC释放的白细胞介素（IL）-6显著增加（p = 0.01）。在第6周，与SO组相比，FO组中PBMC释放的IL-6显著更多（p = 0.01）。通过方差分析，CD-4细胞计数无变化。结论：含FO的食物棒耐受性良好，并允许ω-3脂肪酸的掺入。尽管有证据表明对类花生酸和细胞因子的产生有显著的代谢作用，但在没有进一步研究的情况下，FO在HIV感染患者中的广泛临床使用是不必要的，特别是考虑到在该剂量和使用这种类型的鱼油时CD-4细胞数量有下降的趋势。
Background. Dietary fish oil (FO) has been shown to modulate the immune system. The purpose of this study was to explore the effects of FO supplementation on the production of dienoic eicosanoids and cytokines in patients with human immunodeficiency virus (HIV) infection. Methods: This was a randomized, prospective, double-blind study that included homosexual males with HIV infection. Patients were asked to consume voluntarily five food bars daily containing FO (n = 10) or safflower oil (SO) (n = 9) for 6 weeks. At baseline and week 6, plasma was obtained to measure incorporation of omega-3 fatty acids. At baseline, week 3, and week 6, measurements were made of changes in dienoic eicosanoids and cytokines from lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMC) or spontaneously releasing cells. Results: In the FO group but not the SO group, there was increased incorporation of the omega-3 fatty acid docosahexaenoic acid (DHA) into the phospholipids of the fatty acids of the plasma. In the FO group, there was a significant decrease (p = .01) in 6-keto prostaglandin (PG) F-1alpha released from PBMC. There was a significant increase (p = .01) in interleukin (IL)-6 released from the PBMC in the FO group between baseline and week 3 and between week 3 and week 6. At week 6, there was significantly more IL-6 (p = .01) released from the PBMC in the FO group compared with the SO group. There was no change in CD-4 cell counts by analysis of variance. Conclusions: The FO-containing food bars were well tolerated and allowed incorporation of omega-3 fatty acids to occur. Despite evidence of significant metabolic effects on eicosanoid and cytokine production, widespread clinical use of FO for HIV-infected patients is not warranted without further study, particularly given the trend toward a decline in CD-4 cell numbers at this dose and with this type of fish oil.