CORRELATION OF CELL-SURFACE PHENOTYPE WITH THE ESTABLISHMENT OF INTERLEUKIN 3-DEPENDENT CELL-LINES FROM WILD-MOUSE MURINE LEUKEMIA VIRUS-INDUCED NEOPLASMS
CORRELATION OF CELL-SURFACE PHENOTYPE WITH THE ESTABLISHMENT OF INTERLEUKIN 3-DEPENDENT CELL-LINES FROM WILD-MOUSE MURINE LEUKEMIA VIRUS-INDUCED NEOPLASMS
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DOI：
10.1073/pnas.82.19.6687
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
IHLE, JN
中科院分区：
文献类型：
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作者：
HOLMES, KL;PALASZYNSKI, E;IHLE, JN
野生小鼠嗜生态病毒Cas-Br-M小鼠白血病病毒在NFS小鼠中诱导髓系和红系白血病以及t细胞和b细胞淋巴瘤。在t细胞来源的淋巴因子白介素3 （IL-3）存在或不存在的情况下，这些肿瘤建立长期细胞系的能力被检查。il -3依赖性细胞系可从大多数髓系或红系白血病和免疫母细胞淋巴瘤中获得。在缺乏IL-3的情况下，仅从骨髓性白血病中获得一种长期因子独立细胞系。在IL-3存在或不存在的情况下，大多数胸腺t细胞淋巴瘤或b系淋巴瘤不能培养。结果表明，造血谱系的转化并不一定能消除对正常生长因子的需求。从生长因子中获得独立性可能需要额外的转化事件。
The wild mouse ecotropic virus, Cas-Br-M murine leukemia virus, induces myeloid and erythroid leukemias as well as T-cell and B-cell lymphomas in NFS mice. The ability to establish long-term cell lines from these tumors in the presence or absence of the T-cell-derived lymphokine interleukin 3 (IL-3) was examined. IL-3-dependent cell lines were readily obtained from the majority of the myeloid or erythroid leukemias and immunoblastic lymphomas. In the absence of IL-3, only one long-term factor-independent cell line was obtained from a myelogenous leukemia. The majority of the thymic T-cell lymphomas or B-lineage lymphomas could not be cultured in the presence or absence of IL-3. The results suggest that transformation of hematopoietic lineages does not necessarily obviate the requirement for normal growth factors. The acquisition of independence from growth factors may require additional transforming events.