Microimaging of Oxygen Concentration near Live Photosynthetic Cells by Electron Spin Resonance
Microimaging of Oxygen Concentration near Live Photosynthetic Cells by Electron Spin Resonance
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DOI：
10.1016/j.bpj.2010.05.002
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发表时间：
2010-08-04
影响因子：
3.4
通讯作者：
Blank, Aharon
中科院分区：
文献类型：
--
作者：
Halevy, Revital;Tormyshev, Victor;Blank, Aharon
我们提出了什么是，据我们所知，一种新的方法，高分辨率的三维成像的氧浓度附近的活细胞。将细胞置于稳定的顺磁探针的缓冲溶液中，并采用电子自旋共振显微成像来绘制探针的自旋-自旋弛豫时间（T-2）。这些信息与氧分子的浓度直接相关。该方法是证明与测试样品和少量的活光合细胞（蓝藻），在黑暗和光照的条件下。类似于30 × 30 × 100 Am的空间分辨率被证明，具有类似于μ M氧浓度灵敏度和亚fmol绝对氧灵敏度每体素。使用电子自旋共振显微成像氧映射附近的细胞补充了目前可用的技术的基础上微电极或荧光/磷光。此外，适当的顺磁性探针，它也将很容易适用于细胞内氧显微成像，其他方法发现很难实现的能力。
We present what is, to our knowledge, a new methodology for high-resolution three-dimensional imaging of oxygen concentration near live cells. The cells are placed in the buffer solution of a stable paramagnetic probe, and electron spin-resonance microimaging is employed to map out the probe's spin-spin relaxation time (T-2). This information is directly linked to the concentration of the oxygen molecule. The method is demonstrated with a test sample and with a small amount of live photosynthetic cells (cyanobacteria), under conditions of darkness and light. Spatial resolution of similar to 30 x 30 x 100 Am is demonstrated, with similar to mu M oxygen concentration sensitivity and sub-fmol absolute oxygen sensitivity per voxel. The use of electron spin-resonance microimaging for oxygen mapping near cells complements the currently available techniques based on micro-electrodes or fluorescence/phosphorescence. Furthermore, with the proper paramagnetic probe, it will also be readily applicable for intracellular oxygen microimaging, a capability which other methods find very difficult to achieve.