Localization of SV40 genes within supercoiled loop domains.
Localization of SV40 genes within supercoiled loop domains.
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SV40 基因在超螺旋环域内的定位。
DOI：
10.1093/nar/8.23.5623
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发表时间：
1980
影响因子：
14.9
通讯作者：
Vogelstein,B
中科院分区：
文献类型：
--
作者：
Nelkin,BD;Pardoll,DM;Vogelstein,B
最近的研究表明，真核生物的DNA被组织成超螺旋环结构域。这些环似乎在其基部锚定到不溶性的核骨架或基质上。环中的大多数DNA可以通过核酸酶消化从基质中释放出来;与核基质一起保留的残留DNA代表环的碱基处的序列，以及可能由于其他原因与核基质密切相关的其他序列。使用Southern印迹技术的定量应用，我们已经发现从SV 40感染的3 T3细胞中的残留DNA富集在SV 40序列中，表明它们位于基质DNA附着点附近。在三种不同的SV 40感染的3 T3细胞系中，发现相对于总细胞DNA的3-7倍富集。用珠蛋白基因进行的对照实验表明，在这种残留的基质DNA中没有这种富集。这种序列特异性表明，DNA序列在环内的空间组织可能与这些序列在细胞内的功能有关。
Recent studies indicate that eukaryotic DNA is organized into supercoiled loop domains. These loops appear to be anchored at their bases to an insoluble nuclear skeleton or matrix. Most of the DNA in the loops can be released from the matrix by nuclease digestion; the residual DNA remaining with the nuclear matrix represents sequences at the base of the loops, and possibly other sequences which are intimately associated with the nuclear matrix for other reasons. Using a quantitative application of the Southern blotting technique, we have found this residual DNA from SV4O infected 3T3 cells to be enriched in SV40 sequences, indicating that they reside near matrix-DNA attachment points. An enrichment of 3–7 fold relative to total cellular DNA, was found in each of three different lines of SV40 infected 3T3 cells. Control experiments with globin genes showed no such enrichment in this residual matrix DNA. This sequence specificity suggests that the spatial organization of DNA sequences within loops may be related to the functionality of these sequences within the cell.