Antimicrobial action from a novel porphyrin derivative in photodynamic antimicrobial chemotherapy in vitro
Antimicrobial action from a novel porphyrin derivative in photodynamic antimicrobial chemotherapy in vitro
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DOI：
10.1007/s10103-014-1681-6
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发表时间：
2015-01-01
影响因子：
2.1
通讯作者：
Obana, Akira
中科院分区：
文献类型：
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作者：
Latief, Miftahul Akhyar;Chikama, Taiichiro;Obana, Akira
为确定角膜感染的改进治疗方法所做的努力包括开发光动力学抗微生物化疗（PACT）。我们评估了PACT与新型卟啉衍生物TONS 504和新型光系统对甲氧西林敏感金黄色葡萄球菌（MSSA）和耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌效果。在不同浓度的TONS 504存在下，用发光二极管（LED）以10、20或30 J/cm（2）的能量照射细菌。在照射后30分钟和24小时通过测定琼脂平板上的菌落形成来评估细菌活力。PACT在光照后30 min即可抑制MSSA和MRSA的生长。对于MSSA，在TONS 504浓度为1 mg/L和LED能量为千分之一日元10 J/cm（2）或TONS 504浓度为0.5 mg/L和LED能量为千分之一日元20 J/cm（2）的照射后24 h，细菌生长明显受到完全抑制，对于MRSA，TONS 504浓度为10 mg/L，LED能量为千分之一日元10 J/cm（2），或者TONS 504浓度为1 mg/L，LED能量为千分之一日元20 J/cm（2）。在不存在辐照的情况下，细菌生长不受TONS 504的影响，或者在不存在TONS 504的情况下，细菌生长不受辐照的影响。因此，我们的结果证明了PACT与TONS 504和LED在体外对MSSA和MRSA的抗菌功效，因此，它们为进一步研究该系统作为角膜感染的潜在治疗提供了基础。
Efforts to identify improved treatments for corneal infection include the development of photodynamic antimicrobial chemotherapy (PACT). We evaluated the antimicrobial effect of PACT with a novel porphyrin derivative, TONS 504, and a novel light system on methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA). Bacteria were irradiated with a light-emitting diode (LED) at energies of 10, 20, or 30 J/cm(2) in the presence of various concentrations of TONS 504. Bacterial viability was assessed at 30 min and 24 h after irradiation by determination of colony formation on agar plates. PACT inhibited the growth of both MSSA and MRSA as early as 30 min after light exposure. Complete inhibition of bacterial growth was apparent at 24 h after irradiation at a TONS 504 concentration of 1 mg/L and LED energies of a parts per thousand yen10 J/cm(2) or a TONS 504 concentration of 0.5 mg/L and LED energies of a parts per thousand yen20 J/cm(2) for MSSA, and at a TONS 504 concentration of 10 mg/L and LED energies of a parts per thousand yen10 J/cm(2) or of a TONS 504 concentration of 1 mg/L and LED energies of a parts per thousand yen20 J/cm(2) for MRSA. Bacterial growth was unaffected by TONS 504 in the absence of irradiation or by irradiation in the absence of TONS 504. Our results thus demonstrate the antimicrobial efficacy of PACT with TONS 504 and a LED against both MSSA and MRSA in vitro, and they therefore provide a basis for further investigation of this system as a potential treatment for corneal infection.