Non-specific immune response of healthy and immunocompromised Asian catfish (Clarias batrachus) to several immunostimulants
Non-specific immune response of healthy and immunocompromised Asian catfish (Clarias batrachus) to several immunostimulants
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DOI：
10.1016/j.aquaculture.2005.12.012
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发表时间：
2006-05
期刊：
影响因子：
4.5
通讯作者：
J. Kumari;P. Sahoo
中科院分区：
文献类型：
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作者：
J. Kumari;P. Sahoo
进行本研究以确定四种众所周知的饮食免疫调节剂的功效和免疫调节作用，乳铁蛋白、β-1，3-葡聚糖、左旋咪唑和维生素C对健康和环磷酰胺（CTX）诱导的免疫受损的亚洲胡子鲶（Clarias batrachus）的先天免疫应答的影响。试验分为5组，每组饲喂添加或不添加适当剂量免疫刺激剂的饲料。以200 mg/kg体重的比率连续3天腹膜内注射环磷酰胺，以诱导对照鱼的免疫功能低下状态。注射后72小时，所有组的鱼流血的和先天免疫参数，体液（血凝滴度和总血清髓过氧化物酶含量）和细胞（呼吸爆发和吞噬活性），进行了测定。结果显示，与其各自的对照组相比，作为饲料补充剂递送的所有物质显著（P<0.05）增强了健康和免疫抑制亚组中的大多数非特异性免疫参数。与对照组相比，左旋咪唑诱导健康鱼和免疫功能低下鱼的血液吞噬活性显著增加，如从升高的呼吸爆发和髓过氧化物酶活性观察到的。维生素C的喂养显着提高吞噬活性和髓过氧化物酶含量。另一方面，与各自的对照组相比，两组中的所有参数都受到乳铁蛋白和葡聚糖喂养的积极影响。
The present study was performed to determine the efficacy and immunoreversal effects of four well-known dietary immunomodulators viz., lactoferrin, β-1,3 glucan, levamisole and vitamin C on the innate immune response of healthy and cyclophosphamide (CYP) induced immunocompromised Asian catfish, Clarias batrachus. Fish specimens were divided into 5 groups and each group was fed with diets supplemented with or without an appropriate dose of immunostimulant. Cyclophosphamide at a rate of 200 mg/kg body weight was injected intraperitoneally for 3 consecutive days to induce an immunocompromised state in control fish. After 72 h post injection, fish of all the groups were bled and innate immune parameters, both humoral (haemagglutination titre and total serum myeloperoxidase content) and cellular (respiratory burst and phagocytic activities), were determined. The results showed that all the substances delivered as feed supplements significantly (P<0.05) enhanced most of the non-specific immune parameters in both healthy and immunosuppressed subgroups compared to their respective controls. Levamisole induced significantly increase in blood phagocytic activities in both healthy and immunocompromised fish as observed from raised respiratory burst and myeloperoxidase activity as compared to control. Feeding of vitamin C significantly enhanced phagocytic activity and myeloperoxidase content. On the other hand, all parameters were positively influenced by lactoferrin and glucan feeding in both groups compared to their respective controls.