Lentiviral-Mediated shRNA Silencing of PDE4D Gene Inhibits Platelet-Derived Growth Factor-Induced Proliferation and Migration of Rat Aortic Smooth Muscle Cells.
Lentiviral-Mediated shRNA Silencing of PDE4D Gene Inhibits Platelet-Derived Growth Factor-Induced Proliferation and Migration of Rat Aortic Smooth Muscle Cells.
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慢病毒介导的 shRNA 沉默 PDE4D 基因可抑制血小板衍生生长因子诱导的大鼠主动脉平滑肌细胞的增殖和迁移。
DOI：
10.4061/2011/534257
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发表时间：
2011
影响因子：
1.5
通讯作者：
Sheng W
中科院分区：
文献类型：
--
作者：
Liu L;Xu X;Li J;Li X;Sheng W
磷酸二酯酶4D（PDE 4D）是磷酸二酯酶大超家族的成员。已发现PDE4D多态性与缺血性卒中相关。血管平滑肌细胞（VSMCs）的增殖和迁移在动脉粥样硬化的发病机制中起着关键作用。在本研究中，携带针对PDE 4D的shRNA的慢病毒颗粒感染VSMC显著抑制血小板源性生长因子诱导的VSMC增殖和迁移，并且抑制作用与细胞内cAMP水平无关。我们的研究结果表明，PDE 4D在VSMC增殖和迁移中起重要作用，这可能解释其对缺血性卒中的遗传易感性。
Phosphodiesterase 4D (PDE4D) is a member of the large superfamily of phosphodiesterases. PDE4D polymorphisms have been found to associate with ischemic stroke. Proliferation and migration of vascular smooth muscle cells (VSMCs) play a critical role in the pathogenesis of atherosclerosis. In this study, infection of VSMCs with lentivrius particles carrying shRNA direct against PDE4D significantly inhibited platelet-derived growth factor-induced VSMC proliferation and migration, and the inhibitory effects were not associated with global intracellular cAMP level. Our results implicate that PDE4D has an important role in VSMC proliferation and migration which may explain its genetic susceptibility to ischemic stroke.