Preliminary investigation of crystals of the neutral lipase from Pseudomonas fluorescens.
Preliminary investigation of crystals of the neutral lipase from Pseudomonas fluorescens.
复制标题
荧光假单胞菌中性脂肪酶晶体的初步研究。
DOI：
10.1016/0022-2836(91)90732-l
复制
发表时间：
1991
影响因子：
5.6
通讯作者：
McPherson,A
中科院分区：
文献类型：
--
作者：
Larson,S;Day,J;Greenwood,A;Oliver,J;Rubingh,D;McPherson,A
The neutral lipase from the bacteria Pseudomonas fluorescens, marketed under the trade name LpL-200S, has been crystallized in a form suitable for X-ray diffraction analysis from 35% n-propanol at pH 8· 5. The crystals are monoclinic prisms and are of space group C2 with a= 91· 00 A ̊, b= 47· 17 A ̊, c= 35· 21 A ̊ and β= 121· 43°. There is one molecule of the protein as the asymmetric unit of the crystals. The diffraction pattern extends to at least 1· 6 Å resolution and the crystals are extremely robust in terms of X-ray exposure.
